Biogenic synthesis of iron oxide nanoparticles using Agrewia optiva and Prunus persica phyto species: Characterization, antibacterial and antioxidant activity.

A phytoextract mediated synthesis of iron oxide nanoparticles using Agrewia optiva (Dhaman or Biul) and Prunus persica (Peach) leaf extract as capping and stabilizing agent without using hazardous toxic chemicals via biogenic route has been studied. The biogenic method of synthesis is convenient, rapid, cost effective and ecofriendly. The green synthesized nanoparticles were characterized by Ultraviolet-visible spectroscopy, Fourier transform infrared spectroscopy, Attenuated total reflectance spectroscopy, X-ray diffraction analysis, scanning electron microscopy, energy dispersive X-ray spectroscopy, transmission electron microscopy and dynamic light scattering measurements. The antibacterial study was determined by agar well diffusion method to measure the efficiency of both phyto species extract and its mediated iron oxide nanoparticles against five gram positive bacterial stains such as Staphylococcus aureus (S. aureus), Streptococcus mutans (S. mutans), Streptococcus pyrogenes (S. pyrogenes), Corynebacterium diphtheriae (C. diphtheriae) and Corynebacterium xerosis (C. xerosis) and three gram negative bacterial stains such as Escherichia coli (E. coli), Klebsiella pneuomoniae (K. pneuomoniae) and Pseudomonas aeruginosa (P. aeruginosa). The antibiotic Ciprofloxacin and Gentamicin have been used as reference standard drugs for gram positive and gram negative bacterial stains, respectively. The antioxidant activity of the phyto extracts and prepared nanoparticles have been performed using 2,2-diphenyl-1-picrylhydrazyl (DPPH) free radical assay employing l-ascorbic acid as a standard.

Mirza AU ，Kareem A ，Nami SAA ，Khan MS ，Rehman S ，Bhat SA ，Mohammad A ，Nishat N ... -  《-》

Malus pumila and Juglen regia plant species mediated zinc oxide nanoparticles: Synthesis, spectral characterization, antioxidant and antibacterial studies.

Zinc oxide nanoparticles derived from Malus pumila (apple) and Juglen regia (walnut) plant is an attractive area of research because of their widespread use. The use of plant material to synthesize zinc oxide nanoparticles has been considered as one of the best environmentally friendly approach. This method appears to be low-cost as compare to other conventional method of synthesis. The biosynthesized nanoparticles were characterized by Ultraviolet-visible spectroscopy (UV-visible), Fourier transform infrared spectroscopy (FT-IR), Attenuated total reflectance spectroscopy (ATR), X-ray diffraction (XRD), Scanning electron microscopy (SEM), Energy dispersive X-ray spectroscopy (EDX), Transmission electron Microscopy (TEM) and Dynamic light scattering (DLS). The antioxidant potential has been evaluated by 2, 2-diphenyl-1-picrylhydrazyl free radical (DPPH) assay using L-ascorbic acid as a standard. The antibacterial activity was measured by agar well diffusion method to measure the efficacy of plant species extract and extract mediated zinc oxide nanoparticles against 5 g positive bacteria namely Staphylococcus aureus (S. aureus), Streptococcus mutans (S. mutans), Streptococcus pyrogenes (S. pyrogenes), Corynebacterium diphtheriae (C. diphtheriae) and Corynebacterium xerosis (C. xerosis) and 3 g negative bacteria such as Escherichia coli (E. coli), Klebsiella pneumoniae (K. pneumoniae) and Pseudomonas aeruginosa (P. aeruginosa) bacteria with standard antibiotic for gram positive (Ciprofloxacin) and gram negative bacteria (Gentamicin).

Mirza AU ，Kareem A ，Nami SAA ，Bhat SA ，Mohammad A ，Nishat N ... -  《-》

Ultrasound Assisted Green Synthesis of Silver and Iron Oxide Nanoparticles Using Fenugreek Seed Extract and Their Enhanced Antibacterial and Antioxidant Activities.

Deshmukh AR ，Gupta A ，Kim BS 《-》

Phyto-mediated biosynthesis of silver nanoparticles using the rind extract of watermelon (Citrullus lanatus) under photo-catalyzed condition and investigation of its antibacterial, anticandidal and antioxidant efficacy.

The biological synthesis of nanoparticles has gained tremendous interest, and plants and plant extracts are preferred over other biological sources for this process because of their rich content of bioactive metabolites. In this study, silver nanoparticles (AgNPs) were produced utilizing the aqueous extract of watermelon rind (WRA), an agricultural waste material under photo exposed condition at room temperature, and tested for their antibacterial, anticandidal and antioxidant activities. The synthesized AgNPs showed surface plasmon resonance at 425nm with an average size of 109.97nm. The morphology and elemental composition was confirmed by scanning electron microscopy (SEM) and energy dispersive X-ray analysis (EDX). The Fourier transform infrared spectroscopy (FT-IR) and thermogravimetric and differential thermogravimetric analysis (TG/DTG) confirmed that the bioactive compounds from the WRA extract were involved in the synthesis and capping of AgNPs. X-ray diffraction (XRD) revealed the crystallite nature of the AgNPs. The AgNPs exhibited strong broad spectrum antibacterial activity against five different foodborne bacteria with zones of inhibition 9.12-14.54mm in diameter. When AgNPs were mixed with kanamycin and rifampicin the mixture exhibited strong antibacterial synergistic activity. The AgNPs also exerted strong synergistic anticandidal activity when they were combined with amphotericin b. The AgNPs had high antioxidant activity and reducing power. Overall, the results confirmed the bio-potentials of the synthesized AgNPs using WRA, which could have applications in the biomedical, cosmetic, pharmaceutical, food preservation and packaging industries.

Patra JK ，Das G ，Baek KH 《-》

Photo-catalytic, anti-bacterial, and anti-cancer properties of phyto-mediated synthesis of silver nanoparticles from Artemisia tournefortiana Rchb extract.

Metal nanoparticles have largely been investigated due to their potential medicinal activities. This study demonstrates the biological properties of green-synthesized silver nanoparticles (AgNPs) by using Artemisia tournefortiana Rchb ethanol extract. Instrumentations such as ultraviolet-visible spectra analysis, high-resolution transmission electron microscopy, scanning electron microscopy, energy-dispersive X-ray analysis, X-ray diffraction, and Fourier transform infrared spectroscopy were used to reveal the synthesized AgNPs. Microscopic results showed that the particles were mostly spherical in shape, having an average diameter of 22.89±14.82nm. The antibacterial activity of the phyto-fabricated AgNPs was investigated by the determination of minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC). The in vitro cytotoxicity effect was investigated against normal human embryonic kidney (HEK293) cells and human colon adenocarcinoma cancer (HT29) cells. The apoptotic cells were identified by annexin V/PI FITC staining, and morphological assessment. The expressions of Bax and Bcl2 were evaluated by quantitative real time PCR method. The phyto-synthesized AgNPs have shown increased cell apoptosis and demonstrated dose-dependent cytotoxicity in HT29 cancer cells. Moreover, the photocatalytic activity of the phyto-synthesized AgNPs was evaluated by degradation of Coomassie Brilliant Blue G-250 under UV light exposure and these fabricated Ag nanoparticles demonstrated efficacy in degrading the dye within 60min. Overall, the present results highlighted the antibacterial and anticancer properties of fabricated AgNPs, suggesting that phyto-synthesized silver nanoparticles could possess potent anti-pathogenic bacteria and anti-colon cancer activities.

Baghbani-Arani F ，Movagharnia R ，Sharifian A ，Salehi S ，Shandiz SAS ... -  《-》