Antimicrobial resistance, multilocus sequence types and virulence profiles of ESBL producing and non-ESBL producing uropathogenic Escherichia coli isolated from cats and dogs in Switzerland.

Among 64 uropathogenic Escherichia coli (UPEC) isolated from 13 cats and 51 dogs, 35 were extended-spectrum beta-lactamase (ESBL) producers, and 29 were non-ESBL producers. Forty-six (71.9%) of the isolates were multidrug resistant (MDR). Among the ESBL producers, blaCTX-M-15 (n = 17/48.6% of the blaESBLs), blaCTX-M-1 (n = 10/28.6%), blaCTX-M-55 (n = 4/11.4%), blaCTX-M-14 (n = 3/8.6%), and blaCTX-M-27 (n = 1/2.9%) were identified. The plasmid-mediated fluoroquinolone resistance genes aac(6')-Ib-cr, qnrB and the azithromycin resistance gene mph(A) were detected in 17 (26.6% of all isolates), one (1.6%) and in 13 (20.3%) respectively. The most frequent phylogenetic groups were C (n = 19) and B2 (n = 15). Twenty-six different sequence types (STs) were identified, with two being novel. The most frequent STs were ST410 (n = 16/25%), ST131, and ST73 (both n = 5/7.8%), and ST361 (n = 4/6.3%). Ten (15.6%) of the STs have been associated with urinary tract infection (UTI) in humans, suggesting zoonotic potential. Among seven virulence-associated genes, fyuA was the most prevalent. The overall aggregate virulence factor (VF) score was highest for isolates belonging to phylogenetic group B2 (median aggregate VF score 6, mean score 5,5, range 3-7), and lowest for isolates belonging to phylogenetic group C (0/ 0.5/0-3). The most frequent ST in this study, ST410, harboured the lowest number of VF (0/0,3/0-2). VF scores were higher in NDR (4/3.8/3-4) than in MDR (1/1,9/0-7), and higher in non-ESBL producing isolates (3/3/0-7) than in ESBL producers (1/1,7/0-7). Our data advance our knowledge of the phenotypic and genotypic characteristics of UPEC in companion animals and their potential for infection, zoonotic transmission and dissemination of antimicrobial resistance determinants.

Zogg AL ，Zurfluh K ，Schmitt S ，Nüesch-Inderbinen M ，Stephan R ... -  《-》

ESBL-producing uropathogenic Escherichia coli isolated from dogs and cats in Switzerland.

Extended-spectrum β-lactamase (ESBL)-producing Escherichia (E.) coli have emerged in human and veterinary medicine. The aim of this study was to investigate the presence of ESBL-producers among uropathogenic E. coli isolated from dogs and cats and to characterize detected ESBL-producing isolates by antibiotic susceptibility testing, identification of ESBL genes, multi-locus sequence typing (MLST), detection of putative virulence genes, and analysis of E. coli phylogroups. Among the 107 E. coli isolates derived from urinary samples (59 from dogs, 40 from cats), eight isolates from four different animals (two dogs, two cats) were found to be ESBL-producers. These isolates were of ST533/CTX-M-15/TEM/phylogroup B1 (four strains from one dog), ST410/CTX-M-15/TEM/phylogroup A (three strains, one from a dog and two from a cat), and ST648/CTX-M-15/phylogroup D (one strain from a cat). In terms of putative virulence factors, all isolates harbored lpfA, sat, and tsh, whereas iss was only detected in strains of ST533. Thus, ESBL-producers were detected among uropathogenic E. coli from Swiss companion animals and the eight CTX-M-15-producing isolates belonged to three sequence types (ST410, ST533, ST648) and three E. coli phylogroups (A, B1, D). For the first time, E. coli of ST533 carrying bla(CTX-M-15) were thereby detected in a dog.

Huber H ，Zweifel C ，Wittenbrink MM ，Stephan R ... -  《-》

Veterinary Hospital Dissemination of CTX-M-15 Extended-Spectrum Beta-Lactamase-Producing Escherichia coli ST410 in the United Kingdom.

Timofte D ，Maciuca IE ，Williams NJ ，Wattret A ，Schmidt V ... -  《-》

Spread of CTX-Type Extended-Spectrum β-Lactamase-Producing Escherichia coli Isolates of Epidemic Clone B2-O25-ST131 Among Dogs and Cats in Japan.

Kawamura K ，Sugawara T ，Matsuo N ，Hayashi K ，Norizuki C ，Tamai K ，Kondo T ，Arakawa Y ... -  《-》

Multilocus Sequence Typing and Virulence Profiles in Uropathogenic Escherichia coli Isolated from Cats in the United States.

Liu X ，Thungrat K ，Boothe DM 《PLoS One》