Garcinia xanthochymus extract protects PC12 cells from H(2)O(2)-induced apoptosis through modulation of PI3K/AKT and NRF2/HO-1 pathways.

The aim of the present study was to investigate the protective effects and underlying mechanisms of Garcinia xanthochymus, a perennial medicinal plant native to Yunnan, China, against H2O2-induced oxidative damage in rat pheochromacytoma PC12 cells. Preincubation of PC12 cells with fruit EtOAc fraction (fruit-EFr., 12.5-50 µmol·L-1) of G. xanthochymus for 24 h prior to H2O2 exposure markedly improved cell viability and increased the activities of antioxidant enzymes (superoxide dismutase, catalase, and heme oxygenase-1 [HO-1]), prevented lactate dehydrogenase release and lipid peroxidation malondialdehyde production, attenuated the decrease of matrix metalloproteinases (MMP), and scavenged reactive oxygen species (ROS). Fruit-EFr. also reduced BAX and cytochrome C expression and improved BCL-2 expression, thereby decreasing the ratio of BAX to BCL-2. Fruit-EFr. activated the nuclear translocation of NRF2 to increase HO-1 and induced the phosphorylation of AKT. Its cytoprotective effect was abolished by LY294002, a specific inhibitor of PI3K. Taken together, the above findings suggested that fruit-EFr.of G. xanthochymus could enhance cellular antioxidant defense capacity, at least in part, through upregulating HO-1 expression and activating the PI3K/AKT pathway and that it could suppress H2O2-induced oxidative damage via PI3K/AKT and NRF2/HO-1 signaling pathways.

Xu J ，Gan S ，Li J ，Wand DB ，Chen Y ，Hu X ，Yang GZ ... -  《-》

Resveratrol Attenuates the Cytotoxicity Induced by Amyloid-β(1-42) in PC12 Cells by Upregulating Heme Oxygenase-1 via the PI3K/Akt/Nrf2 Pathway.

Hui Y ，Chengyong T ，Cheng L ，Haixia H ，Yuanda Z ，Weihua Y ... -  《-》

Klotho protein inhibits H(2)O(2)-induced oxidative injury in endothelial cells via regulation of PI3K/AKT/Nrf2/HO-1 pathways.

Cui W ，Leng B ，Wang G 《-》

The cytoprotective effects of 7,8-dihydroxyflavone against oxidative stress are mediated by the upregulation of Nrf2-dependent HO-1 expression through the activation of the PI3K/Akt and ERK pathways in C2C12 myoblasts.

Kang JS ，Choi IW ，Han MH ，Kim GY ，Hong SH ，Park C ，Hwang HJ ，Kim CM ，Kim BW ，Choi YH ... -  《-》

Rhein from Rheum rhabarbarum Inhibits Hydrogen-Peroxide-Induced Oxidative Stress in Intestinal Epithelial Cells Partly through PI3K/Akt-Mediated Nrf2/HO-1 Pathways.

Zhuang S ，Yu R ，Zhong J ，Liu P ，Liu Z ... -  《-》