Potential circulating miRNA signature for early detection of NSCLC.

Circulating microRNAs (c-miRNAs) are promising biomarkers for screening, early detection and prognosis of cancer. The purpose of this investigation was to identify a panel of c-miRNAs in plasma that could contribute to early detection of non-small cell lung cancer (NSCLC). We profiled the expression of 44 unique plasma miRNAs in training set of 34 NSCLC patients and 20 matched healthy individuals by miRCURY LNA™ Universal RT microRNA PCR Panel and calculated dysregulation fold changes using the 2-ΔΔCt equation. Selected plasma miRNAs were then validated by SYBR green q-RT PCR using an independent validation set of plasma samples from NSCLC patients (n: 72) and NC (n: 50). In the validation set, the receiver operating characteristic (ROC) curves were generated for four miRNAs. In the training set, 17 miRNAs were significantly up-regulated and nine were down-regulated in the plasma from NSCLC patients versus matched normal controls. Four miRNAs (miR-21, miR-328, miR-375 and miR-141) were selected for validating their diagnostic value in the testing set. ROC plot analysis showed that a high specificity (98%) and sensitivity (82.7%) in miR-141 in comparing early NSCLC patient and controls. So among these four plasma miRNAs only miR-141 could be promising biomarkers for early detection of NSCLC. In addition to, we found a significant positive correlation between stage and miR-21 expression level (95% CI: 0.687-0.863; p-value < 0.0001). Considering the accessibility and stability of circulating miRNAs, plasma miR-141 is a useful biomarker early detection of NSCLC as a supplement in future screening studies.

Arab A ，Karimipoor M ，Irani S ，Kiani A ，Zeinali S ，Tafsiri E ，Sheikhy K ... -  《Cancer Genetics》

Five microRNAs in plasma as novel biomarkers for screening of early-stage non-small cell lung cancer.

Geng Q ，Fan T ，Zhang B ，Wang W ，Xu Y ，Hu H ... -  《RESPIRATORY RESEARCH》

Identification of serum miRNAs by nano-quantum dots microarray as diagnostic biomarkers for early detection of non-small cell lung cancer.

Fan L ，Qi H ，Teng J ，Su B ，Chen H ，Wang C ，Xia Q ... -  《-》

A unique set of 6 circulating microRNAs for early detection of non-small cell lung cancer.

Halvorsen AR ，Bjaanæs M ，LeBlanc M ，Holm AM ，Bolstad N ，Rubio L ，Peñalver JC ，Cervera J ，Mojarrieta JC ，López-Guerrero JA ，Brustugun OT ，Helland Å ... -  《Oncotarget》

miR-1254 and miR-574-5p: serum-based microRNA biomarkers for early-stage non-small cell lung cancer.

Foss KM ，Sima C ，Ugolini D ，Neri M ，Allen KE ，Weiss GJ ... -  《-》