Circulating Antibodies against Thrombospondin Type-I Domain-Containing 7A in Chinese Patients with Idiopathic Membranous Nephropathy.

Thrombospondin type-I domain-containing 7A (THSD7A) was recently identified as the target antigen in about 10% of patients with M-type phospholipase A2 receptor (PLA2R)-negative membranous nephropathy in European and North American populations. The prevalence of THSD7A in other populations and their clinical associations deserve further clarification. Immunofluorescence assay was performed to investigate anti-THSD7A antibodies in 578 consecutive patients with biopsy-proven idiopathic membranous nephropathy, 114 patients with secondary membranous nephropathy, 64 disease controls, and 20 healthy controls. Glomerular expression of THSD7A antigen was examined by immunohistochemistry. Anti-PLA2R antibodies and glomerular PLA2R expression were also screened. Among the 578 patients with idiopathic membranous nephropathy, 12 (2%) patients were identified as THSD7A-positive: ten patients were THSD7A-positive alone, which accounted for 16% (ten of 64) of PLA2R-negative patients; two patients were dual-positive for both anti-THSD7A and anti-PLA2R antibodies and showed enhanced expression of both antigens colocalized in glomeruli. Among the 114 patients with secondary membranous nephropathy, one among 44 (2%) patients with cancer had anti-THSD7A antibodies, whereas 18 of 44 (41%) had anti-PLA2R antibodies. No anti-THSD7A antibody was detected in other disease controls or healthy individuals. Clinical features were comparable between the patients with and without THSD7A. During follow-up, two patients who achieved remission had a clearance of circulating antibodies against THSD7A, whereas antibodies increased in parallel with proteinuria in a patient with a relapse. THSD7A-associated membranous nephropathy has a low prevalence in Chinese patients. The double-positive patients suggest dual autoimmune responses.

Wang J ，Cui Z ，Lu J ，Probst C ，Zhang YM ，Wang X ，Qu Z ，Wang F ，Meng LQ ，Cheng XY ，Liu G ，Debiec H ，Ronco P ，Zhao MH ... -  《-》

Clinical and Histological Features of Phospholipase A2 Receptor-Associated and Thrombospondin Type-I Domain-containing 7A-Associated Idiopathic Membranous Nephropathy: A Single Center Retrospective Study from China.

Zhang D ，Zou J ，Zhang C ，Zhang W ，Lin F ，Jiang G ... -  《MEDICAL SCIENCE MONITOR》

Circulating antibodies against M-type phospholipase A2 receptor and thrombospondin type-1 domain-containing 7A in Chinese patients with membranous nephropathy.

Tian C ，Li L ，Liu T ，Qu X ，Qiu Y ... -  《-》

Thrombospondin type-1 domain-containing 7A in idiopathic membranous nephropathy.

Tomas NM ，Beck LH Jr ，Meyer-Schwesinger C ，Seitz-Polski B ，Ma H ，Zahner G ，Dolla G ，Hoxha E ，Helmchen U ，Dabert-Gay AS ，Debayle D ，Merchant M ，Klein J ，Salant DJ ，Stahl RAK ，Lambeau G ... -  《-》

Biomarkers to detect membranous nephropathy in Chinese patients.

Lin L ，Wang WM ，Pan XX ，Xu J ，Gao CN ，Zhang W ，Ren H ，Xie JY ，Shen PY ，Xu YW ，Ni LY ，Chen N ... -  《Oncotarget》