Electroacupuncture improves neurobehavioral function and brain injury in rat model of intracerebral hemorrhage.

Acupuncture has been widely used as a treatment for stroke in China for a long time. Recently, studies have demonstrated that electroacupuncture (EA) can accelerate intracerebral hemorrhage (ICH)-induced angiogenesis in rats. In the present study, we investigated the effect of EA on neurobehavioral function and brain injury in ICH rats. ICH was induced by stereotactic injection of collagenase type I and heparin into the right caudate putamen. Adult ICH rats were randomly divided into the following three groups: model control group (MC), EA at non-acupoint points group (non-acupoint EA) and EA at Baihui and Dazhui acupoints group (EA). The neurobehavioral deficits of ICH rats were assessed by modified neurological severity score (mNSS) and gait analysis. The hemorrhage volume and glucose metabolism of hemorrhagic foci were detected by PET/CT. The expression levels of MBP, NSE and S100-B proteins in serum were tested by ELISA. The histopathological features were examined by haematoxylin-eosin (H&E) staining. Apoptosis-associated proteins in the perihematomal region were observed by immunohistochemistry. EA treatment significantly promoted the recovery of neurobehavioral function in ICH rats. Hemorrhage volume reduced in EA group at day 14 when compared with MC and non-acupoint EA groups. ELISA showed that the levels of MBP, NSE and S100-B in serum were all down-regulated by EA treatment. The brain tissue of ICH rat in the EA group was more intact and compact than that in the MC and non-acupoint groups. In the perihematomal regions, the expression of Bcl-2 protein increased and expressions of Caspase-3 and Bax proteins decreased in the EA group vs MC and non-acupoint EA groups. Our data suggest that EA treatment can improve neurobehavioral function and brain injury, which were likely connected with the absorption of hematoma and regulation of apoptosis-related proteins.

Zhu Y ，Deng L ，Tang H ，Gao X ，Wang Y ，Guo K ，Kong J ，Yang C ... -  《-》

Electroacupuncture Enhance Therapeutic Efficacy of Mesenchymal Stem Cells Transplantation in Rats With Intracerebral Hemorrhage.

Previous studies have showed the beneficial effects of mesenchymal stem cells (MSCs) on experimental intracerebral hemorrhage (ICH) animal. Enhancement of the treatment efficacy of MSCs in ICH is essential, considering the diseases association with high rates of disability and mortality. Some auxiliary methods to enhance the beneficial efficacy of MSCs have been introduced. However, the effect of electroacupuncture (EA) on the therapeutic efficacy of MSCs transplantation in hemorrhagic stroke and its potential mechanism is not explored. ICH rat models were established using collagenase and heparin. 48 h after ICH induction, the rats were randomly divided into model control (MC), MSCs transplantation (MSCs), EA stimulation (EA) and MSCs transplantation combined with EA stimulation (MSCs + EA) groups. We used mNSS test and gait analysis to assess neurological function of rats, and PET/CT to evaluate the volume of hemorrhage focus and level of glucose uptake. The concentrations of MDA, SOD, NSE, S100B and MBP in serum or plasma were examined with ELISA. Neural differentiation of MSCs, and the expressions of Bcl-2, Bax, Arg-1 and iNOS proteins around hematoma were detected by immunofluorescence and immunohistochemistry staining respectively. Western blot was carried out to analyze the expression levels of COX4, OGDH, PDH-E1α, Bcl-2 and Bax proteins. TUNEL staining was used to estimate cell apoptosis and transmission electron microscopy (TEM) was used to observe the ultrastructure and number of mitochondria. Our data showed that EA promoted neuron-like differentiation of transplanted MSCs and the expressions of BDNF and NGF proteins in ICH rats. The score of mNSS and the gait analysis showed that the recovery of the neurological function in the MSCs + EA group was better than that in the MSCs and EA groups. EA improved the structure of brain tissue, and alleviated brain injury further after MSCs transplantation in ICH rats. When compared with the MSCs and EA groups, the level of glucose uptake and numbers of mitochondria and Arg-1 positive cells in MSCs + EA group increased significantly, but the numbers of apoptotic cells and iNOS positive cells and volume of hemorrhage focus reduced. The expressional levels of COX4, OGDH, PDH-E1α and Bcl-2 proteins increased, while the expressional level of Bax protein decreased compared with those in the MSCs and EA groups. Our results reveal that EA improve therapeutic efficacy of MSCs transplantation in ICH rats.

Deng L ，Zhou L ，Zhu Y ，Fan G ，Tang H ，Zheng Y ，Gao X ，Guo K ，Zhou P ，Yang C ... -  《-》

Electroacupuncture at GV20‑GB7 regulates mitophagy to protect against neurological deficits following intracerebral hemorrhage via inhibition of apoptosis.

Guan R ，Li Z ，Dai X ，Zou W ，Yu X ，Liu H ，Chen Q ，Teng W ，Liu P ，Liu X ，Dong S ... -  《-》

Electroacupuncture improves recovery after hemorrhagic brain injury by inducing the expression of angiopoietin-1 and -2 in rats.

Zhou HJ ，Tang T ，Zhong JH ，Luo JK ，Cui HJ ，Zhang QM ，Zhou JH ，Zhang Q ... -  《-》

The effects of acupuncture and traditional Chinese medicines on apoptosis of brain tissue in a rat intracerebral hemorrhage model.

This study aimed to evaluate the effects of Chinese herbs and acupuncture on the neuronal apoptosis and the expression of apoptosis-related genes in the brain tissue of rats following intracerebral hemorrhage (ICH). Collagenase VII was injected into the caudate nucleus of Sprague-Dawley rats to induce ICH. Chinese herbs (musk, borneol and tetrandrine) were intragastrically administered, and acupuncture was performed using Dazhui, Fengfu and Shuigou acupoints. Each group was further subdivided into 4 subgroups based on treatment duration (6-hour, 24-hour, 72-hour, and 1-week). Neurological impairment score, TUNEL assay and apoptotic markers, BCL-2, BAX, and caspase-3 were used to evaluate the apoptosis status after ICH and subsequent treatment. Chinese herbal therapy and acupuncture improved neurological impairment compared with no therapy and sham-operated animals. Significant differences in TUNEL positive cells were found between treatment groups (p < 0.001) and over time (p < 0.001). Differential expression of BCL-2, BAX, and caspase-3 was observed between treatment groups (p = 0.014 for BAX and < 0.05 for BCL-2 and caspase-3) and treatment duration groups (p = 0.006 for BAX and < 0.05 BCL-2 and caspase-3). Results indicate that Chinese herbs and acupuncture may improve neural impairment and reduce apoptosis, although there was no difference between therapies in a rat model of ICH. Additional experiments are needed to further clarify the role of these therapies following ICH.

Chen JC 《-》