Blautia massiliensis sp. nov., isolated from a fresh human fecal sample and emended description of the genus Blautia.

The strain GD9T is the type strain of the newly proposed species Blautia massiliensis sp. nov., belonging to the family Lachnospiraceae. It was isolated from a fresh stool sample collected from a healthy human using the culturomics strategy. Cells are Gram-negative rods, oxygen intolerant, non-motile and non-spore forming. The 16S rRNA gene sequencing showed that strain GD9T was closely related to Blautia luti, with a 97.8% sequence similarity. Major fatty acids were C14:0 (19.8%) and C16:0 (53.2%). Strain GD9T exhibits a genome of 3,717,339 bp that contains 3,346 protein-coding genes and 81 RNAs genes including 63 tRNAs. The features of this organism are described here, with its complete genome sequence and annotation. Compared with other Blautia species which are Gram positive, the strain was Gram negative justifying an emended description of the genus Blautia.

Durand GA ，Pham T ，Ndongo S ，Traore SI ，Dubourg G ，Lagier JC ，Michelle C ，Armstrong N ，Fournier PE ，Raoult D ，Million M ... -  《-》

Blautia hominis sp. nov., isolated from human faeces.

Shin NR ，Kang W ，Tak EJ ，Hyun DW ，Kim PS ，Kim HS ，Lee JY ，Sung H ，Whon TW ，Bae JW ... -  《-》

Description and genomic characterization of Massiliimalia massiliensis gen. nov., sp. nov., and Massiliimalia timonensis gen. nov., sp. nov., two new members of the family Ruminococcaceae isolated from the human gut.

Afouda P ，Traore SI ，Dione N ，Andrieu C ，Tomei E ，Richez M ，Di Pinto F ，Lagier JC ，Dubourg G ，Raoult D ，Fournier PE ... -  《-》

Noncontiguous finished genome sequence and description of Intestinimonas massiliensis sp. nov strain GD2(T) , the second Intestinimonas species cultured from the human gut.

Intestinimonas massiliensis sp. nov strain GD2T is a new species of the genus Intestinimonas (the second, following Intestinimonas butyriciproducens gen. nov., sp. nov). First isolated from the gut microbiota of a healthy subject of French origin using a culturomics approach combined with taxono-genomics, it is strictly anaerobic, nonspore-forming, rod-shaped, with catalase- and oxidase-negative reactions. Its growth was observed after preincubation in an anaerobic blood culture enriched with sheep blood (5%) and rumen fluid (5%), incubated at 37°C. Its phenotypic and genotypic descriptions are presented in this paper with a full annotation of its genome sequence. This genome consists of 3,104,261 bp in length and contains 3,074 predicted genes, including 3,012 protein-coding genes and 62 RNA-coding genes. Strain GD2T significantly produces butyrate and is frequently found among available 16S rRNA gene amplicon datasets, which leads consideration of Intestinimonas massiliensis as an important human gut commensal.

Afouda P ，Durand GA ，Lagier JC ，Labas N ，Cadoret F ，Armstrong N ，Raoult D ，Dubourg G ... -  《-》

Microbial Culturomics Application for Global Health: Noncontiguous Finished Genome Sequence and Description of Pseudomonas massiliensis Strain CB-1(T) sp. nov. in Brazil.

Bardet L ，Cimmino T ，Buffet C ，Michelle C ，Rathored J ，Tandina F ，Lagier JC ，Khelaifia S ，Abrahão J ，Raoult D ，Rolain JM ... -  《-》