Reduced expression of microRNA-497 is associated with greater angiogenesis and poor prognosis in human gliomas.

MicroRNA (miR)-497 plays a tumor-suppressive role in several malignancies and is involved in glioma invasiveness and resistance to chemotherapy. To add to the knowledge of the clinical significance of miR-497 in human gliomas, quantitative real-time polymerase chain reaction was performed to detect the expression of miR-497 in 110 pairs of freshly prepared glioma and nonneoplastic brain tissues. Then the associations of miR-497 expression with various clinicopathological characteristics and overall survival of glioma patients were estimated statistically. Gain-of-function assays were also performed to examine the role of miR-497 in glioma angiogenesis. The expression of miR-497 in human glioma tissues was significantly lower than in nonneoplastic brain tissues (P<.001). In addition, low miR-497 expression was significantly associated with advanced World Health Organization grade (P<.001) and low Karnofsky performance scores (P=.02). Moreover, the survival of glioma patients with low miR-497 expression was dramatically shorter than that of patients with high miR-497 expression (P=.001). Forced expression of miR-497 in glioma cells inhibited tube formation by cocultured human brain microvascular endothelial cells. We also found that miR-497 overexpression in glioma cells led to decreased expression of vascular endothelial growth factor. In conclusion, miR-497 may be a favorable prognostic marker in human gliomas, in part by being a negative regulator of angiogenesis, implying its potential as a therapeutic target for this cancer.

Feng F ，Kuai D ，Wang H ，Li T ，Miao W ，Liu Y ，Fan Y ... -  《-》

Up-regulation of microRNA-15b correlates with unfavorable prognosis and malignant progression of human glioma.

Pang C ，Guan Y ，Zhao K ，Chen L ，Bao Y ，Cui R ，Li G ，Wang Y ... -  《International Journal of Clinical and Experimental Pathology》

MicroRNA-203 down-regulation is associated with unfavorable prognosis in human glioma.

MicroRNA-203 (miR-203) serves as a tumor suppressor or a tumor promoter in different human malignancies. However, its involvement in human gliomas is still unclear. The aim of this study was to investigate the clinical significance of miR-203 expression in gliomas. Real-time quantitative PCR was employed to measure the expression level of miR-203 in clinical glioma tissues. The expression of miR-203 was reduced in high WHO grade glioma tissues compared with that in low WHO grade and normal brain tissues, and decreased with ascending tumor WHO grades (P < 0.001). The reduced miR-203 expression in gliomas was significantly associated with higher WHO grade (P < 0.001), lower KPS score (P = 0.008) and poorer disease-specific survival of patients (P = 0.001). More importantly, subgroup analyses according to tumor WHO grade revealed that the disease-specific survival of patients with low miR-203 expression in high WHO grades (III-IV) subgroup was significantly shorter than those with high miR-203 expression (P < 0.001), but no significant difference was found for patients in WHO grades I-II subgroup (P = 0.08). Our data validate an important clinical significance of miR-203 in gliomas, and reveal that it might be an intrinsic regulator of tumor progression and a potential prognostic factor for this dismal disease.

He J ，Deng Y ，Yang G ，Xie W ... -  《-》

Downregulation of microRNA-504 is associated with poor prognosis in high-grade glioma.

Guan Y ，Chen L ，Bao Y ，Pang C ，Cui R ，Li G ，Liu J ，Wang Y ... -  《International Journal of Clinical and Experimental Pathology》

Downregulation of miR-154 in human glioma and its clinicopathological and prognostic significance.

Wang L ，Wu L ，Wu J 《-》