Combined nitrogen limitation and hydrogen peroxide treatment enhances neutral lipid accumulation in the marine diatom Phaeodactylum tricornutum.

Exogenous application of dilute hydrogen peroxide (H2O2) increases neutral lipid production in Phaeodactylum tricornutum. Exposing early stationary phase cultures of P. tricornutum to 0.25-2mM H2O2 increases the amount of neutral lipids per biomass (mg/mg) by >100% at 24h post H2O2 treatment as determined upon lipid extraction and analysis using a neutral lipid assay. H2O2 treatment increased the total levels of neutral lipids harvested up to 50%, from 64mg/L to 96mg/L, demonstrating its possible effectiveness as a pre-harvest strategy to enhance the biofuel feedstock potential of P. tricornutum. The effects of H2O2 on biomass are concentration dependent; increasing concentrations of H2O2 reduce the levels of isolated biomass. Analysis of combined stressors demonstrates that H2O2 treatment exhibits synergistic effects to enhance neutral lipid production under nitrogen-depleted, but not phosphorus-depleted conditions, suggesting that the effects of hydrogen peroxide on lipid production are influenced by environmental nitrogen levels.

Burch AR ，Franz AK 《-》

Photo-Oxidative Stress-Driven Mutagenesis and Adaptive Evolution on the Marine Diatom Phaeodactylum tricornutum for Enhanced Carotenoid Accumulation.

Yi Z ，Xu M ，Magnusdottir M ，Zhang Y ，Brynjolfsson S ，Fu W ... -  《Marine Drugs》

Using a mixture of wastewater and seawater as the growth medium for wastewater treatment and lipid production by the marine diatom Phaeodactylum tricornutum.

This study was conducted to evaluate the potential of the marine diatom Phaeodactylum tricornutum in nutrient removal coupled with biodiesel production using different ratios of mixed municipal wastewater (MW) and seawater (SW) as the growth medium. The results indicated that P. tricornutum exhibited high nutrient removal efficiency with the ratios of MW: SW = 1:1 and MW: SW = 2:1, e.g. 87.7-89.9% for chemical oxygen demand (COD), 82.2-86.7% for total nitrogen (TN), 96.0-97.0% for total phosphorus, and 76.9-84.2% for ammonium (NH3-N). Significantly higher biomass and lipid productivity were obtained with aeration. The highest lipid productivity of P. tricornutum was 54.76 mg/L/day, which was obtained with a two-step cultivation using the ratio of MW: SW = 1:1 by diluting half of the mixture and bubbling with 5% CO2 during the second step. These results suggested that the marine diatom P. tricornutum exhibited great potential for using mixed wastewater for wastewater treatment and biodiesel production.

Wang XW ，Huang L ，Ji PY ，Chen CP ，Li XS ，Gao YH ，Liang JR ... -  《-》

Two marine natural products, penicillide and verrucarin J, are identified from a chemical genetic screen for neutral lipid accumulation effectors in Phaeodactylum tricornutum.

Yu M ，Chen X ，Jiang M ，Li X ... -  《-》

Role of sufficient phosphorus in biodiesel production from diatom Phaeodactylum tricornutum.

Yu SJ ，Shen XF ，Ge HQ ，Zheng H ，Chu FF ，Hu H ，Zeng RJ ... -  《-》