TALEN- and CRISPR-enhanced DNA homologous recombination for gene editing in zebrafish.

The TALE nuclease and clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated systems (Cas) have been developed as important tools for genome editing in zebrafish. Here we describe a CRISPR/Cas9-based approach for generating site-specific gene targeting in zebrafish using DNA double-strand breaks induced homologous recombination (HR)-dependent repair mechanism. Through comicroinjection of Cas9 mRNA, guide RNA targeting genomic DNA sequence corresponding to the twist2 gene and the corresponding double-strand long arm donor template with a point mutation identified in human, HR-mediated knock-in of the expected targeting sequence was obtained. To facilitate identification of germline transmission of targeted mutation, a method of screening sperms of male founder fish is designed.

Zhang Y ，Huang H ，Zhang B ，Lin S ... -  《Methods in Cell Biology》

Knock-In Strategy for Editing Human and Zebrafish Mitochondrial DNA Using Mito-CRISPR/Cas9 System.

Bian WP ，Chen YL ，Luo JJ ，Wang C ，Xie SL ，Pei DS ... -  《ACS Synthetic Biology》

TALEN-Mediated Mutagenesis and Genome Editing.

Ma AC ，Chen Y ，Blackburn PR ，Ekker SC ... -  《-》

Exogenous gene integration mediated by genome editing technologies in zebrafish.

Morita H ，Taimatsu K ，Yanagi K ，Kawahara A ... -  《-》

Site-Specific Integration of Exogenous Genes Using Genome Editing Technologies in Zebrafish.

Kawahara A ，Hisano Y ，Ota S ，Taimatsu K ... -  《INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES》