Raoultella sp. strain L03 fixes N2 in association with micropropagated sugarcane plants.

N2 -fixing bacteria belonging to the genus Raoultella of the family Enterobacteriaceae are widely associated with plants. Raoultella sp. strain L03 was isolated from surface-sterilized sugarcane roots. In this study, we inoculated the strain L03 to microbe-free micropropagated plantlets of the main sugarcane cultivar ROC22 grown in Guangxi, China and determined N2 -fixation and association between strain L03 and sugarcane plants. Inoculation of strain L03 increased plant biomass, total N, N concentration and chlorophyll, and relieved N-deficiency symptoms of plants under an N-limiting condition. An (15) N isotope dilution assay revealed (15) N isotope dilution in the inoculated sugarcane plants and incorporation of the fixed (14) N from air into chlorophyll. Moreover, a gfp-tagged and antibiotic-resistant L03 strain was reisolated from surface-sterilized sugarcane plants and was detected in plant tissues by fluorescent microscopy. This study for the first time demonstrates that a Raoultella bacterium is able to fix N2 in association with the plant host.

Luo T ，Ou-Yang XQ ，Yang LT ，Li YR ，Song XP ，Zhang GM ，Gao YJ ，Duan WX ，An Q ... -  《-》

The actinobacterium Microbacterium sp. 16SH accepts pBBR1-based pPROBE vectors, forms biofilms, invades roots, and fixes N₂ associated with micropropagated sugarcane plants.

Lin L ，Guo W ，Xing Y ，Zhang X ，Li Z ，Hu C ，Li S ，Li Y ，An Q ... -  《-》

Plant growth-promoting nitrogen-fixing enterobacteria are in association with sugarcane plants growing in Guangxi, China.

Lin L ，Li Z ，Hu C ，Zhang X ，Chang S ，Yang L ，Li Y ，An Q ... -  《-》

N-fertilizer saving by the inoculation of Gluconacetobacter diazotrophicus and Herbaspirillum sp. in micropropagated sugarcane plants.

Muthukumarasamy R ，Govindarajan M ，Vadivelu M ，Revathi G ... -  《-》

Diversity of nitrogen-fixing rhizobacteria associated with sugarcane: a comprehensive study of plant-microbe interactions for growth enhancement in Saccharum spp.

Singh RK ，Singh P ，Li HB ，Song QQ ，Guo DJ ，Solanki MK ，Verma KK ，Malviya MK ，Song XP ，Lakshmanan P ，Yang LT ，Li YR ... -  《-》