Microbiological quality of fresh produce obtained from retail stores on the Eastern Shore of Maryland, United States of America.

The aim of this study was to investigate the microbiological quality of six types of fresh produce obtained from three retail stores located on the Eastern Shore of Maryland, USA. A total of 414 samples representing basil, cilantro, lettuce, scallion, spinach, and parsley were analyzed for total aerobic bacteria (APC), total coliforms, Escherichia coli, and three pathogenic bacteria (E. coli O157:H7, Listeria monocytogenes, and Salmonella), using standard methods. Presumptive pathogenic isolates were confirmed using BAX Polymerase Chain Reaction. Total aerobic populations varied widely between samples, while 38.41% were positive for total coliforms and only 10.15% for E. coli. Median abundance (log CFU/g) of total coliforms and E. coli were less than the limit of detection and that of APC ranged from 5.78 to 6.61 over the six produce types. There was a statistically significant difference in prevalence of total coliforms among the retail stores, but not for abundance of APC or prevalence of E. coli. E. coli O157:H7 and L. monocytogenes were detected in one spinach sample each, while one parsley and one cilantro sample were positive for Salmonella. There were no statistically significant differences in microbiological quality among produce types. Although the results of this study provided some indices of sanitary and/or spoilage level, no relationship was observed among the total aerobic bacteria, total coliforms, E. coli, and the presence of pathogenic bacteria in the samples tested.

Korir RC ，Parveen S ，Hashem F ，Bowers J ... -  《-》

Evaluation of a polymerase chain reaction-based system for detection of Salmonella enteritidis, Escherichia coli O157:H7, Listeria spp., and Listeria monocytogenes on fresh fruits and vegetables.

Shearer AE ，Strapp CM ，Joerger RD 《JOURNAL OF FOOD PROTECTION》

Microbiological Quality and Safety of Fresh Fruits and Vegetables at Retail Levels in Korea.

The aim of this study was to evaluate the microbiological quality and safety of fresh produce at retail level in Korea in order to periodically update information and establish available risks associated with consumption of fresh fruits and vegetables. The samples from different markets located in 3 provinces of South Korea were collected. The protocol in the Korean Food Standards Codex was applied and generic Escherichia coli, coliforms, aerobic mesophilic bacteria (AMB), and yeast and mold (YM) in 360 packaged and unpackaged fresh fruits and vegetables were analyzed. Presence of pathogens was examined using real-time polymerase chain reaction (q-PCR) after enrichment of samples. For all, the microbial counts ranged from 1.7 to 10.6 log cfu/g for AMB, 2.2 to 7.9 log cfu/g for coliforms, and 5.5 to 7.9 log cfu/g for YM. Three lettuce samples were contaminated by E. coli with a bacterial load ranging from 2 to 4 log cfu/g. Salmonella spp. were not detected in any fresh produce. Listeria monocytogenes, E. coli O157:H7, and Staphylococcus aureus were found in 1 (0.6%), 3 (0.8%), and 5 (1.4%) fresh produce samples, respectively. Bacillus cereus (50.3%) and Clostridium perfringens (13.3%) had the highest prevalence. These results indicate the need for employing strict control measures and developing preventive strategies to improve the quality and safety of fresh produce in Korea.

Tango CN ，Wei S ，Khan I ，Hussain MS ，Kounkeu PN ，Park JH ，Kim SH ，Oh DH ... -  《-》

Microbiological safety of ready-to-eat fresh-cut fruits and vegetables sold on the Canadian retail market.

Following implementation of Health Canada's Policy on Listeria monocytogenes in Ready-to-Eat Foods by Canadian food safety authorities in 2011, a four-year study (2012-2016) was carried out to gain baseline information on the occurrence of bacterial pathogens, notably the prevalence and levels of Listeria monocytogenes (L. monocytogenes) in various product types of ready-to-eat (RTE) fresh-cut fruits and fresh-cut vegetables sold at retail in Canada. A total of 10,070 pre-packaged samples, including 4691 fresh-cut fruit and 5379 fresh-cut vegetable samples were collected from retail stores across Canada and analyzed for bacterial pathogens and generic Escherichia coli (E. coli). Salmonella species (spp.), E. coli O157:H7, Shigella and Campylobacter were not detected in any of the tested samples. L. monocytogenes was identified in 0.51% (95% CI [0.34, 0.76]) of the fresh-cut fruit and in 0.24% (95% CI [0.14, 0.41]) of the fresh-cut vegetable samples. Of the 37 L. monocytogenes positive samples identified, levels of L. monocytogenes <5 CFU/g, 5-<100 CFU/g, and ≥100 CFU/g were found in 67.6% (25/37), 24.3% (9/37) and 8.1% (3/37) of the samples, respectively. The results of this study indicate that the vast majority of fresh-cut fruits and vegetables sold on the Canadian retail market are safe for consumption. However, contamination by L. monocytogenes can infrequently occur in fresh-cut fruits and vegetables, with certain types of fresh-cut fruits (i.e., melons, apples) and vegetables (i.e., mushrooms, cauliflower) being more likely to be contaminated than others. Safe handling practices are recommended for producers, retailers and consumers including storage at refrigerated temperatures.

Zhang H ，Yamamoto E ，Murphy J ，Locas A ... -  《-》

A field study of the microbiological quality of fresh produce.

Johnston LM ，Jaykus LA ，Moll D ，Martinez MC ，Anciso J ，Mora B ，Moe CL ... -  《JOURNAL OF FOOD PROTECTION》