Comparative transcriptome analysis of Bombyx mori spinnerets and Filippi's glands suggests their role in silk fiber formation.

The spinneret is located at the end of silk glands in the silkworm, Bombyx mori. The Filippi's gland (FG), which communicates with the silk gland, is considered an accessory to the latter. Although these two organs have been known for centuries, only their morphology has been studied. Their gene expression profiles and physiological roles are still unknown. Aided by next-generation sequencing, we profiled more than 11000 transcripts from the spinneret and FG of silkworm larvae on day 3 of the fifth instar (L5D3) and wandering stage (W1) in this study. A total of 59 ion-transporting protein genes and 106 cuticle protein genes were identified in the spinneret. To analyze the dynamic changes in the expression of spinneret genes, differential expression analysis was performed, and 1452 differentially expressed genes (DEGs) were identified in spinneret tissue harvested on L5D3 and W1 of the silkworm larvae. Gene Ontology (GO) enrichment and Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment of the DEGs revealed that the spinneret had active ion-transporting, chitin binding, and energy metabolism processes at W1. Based on these data, we hypothesized that the role of the spinneret is to provide a favorable physiological environment for the silk fiber formation. Furthermore, differential expression analysis and GO enrichment of the DEGs in the FG suggested a possible role of this gland in transporting small solutes such as ions, sugars and amino acids to the silk gland. Our findings pave a way for further functional explanation of the spinneret and FG.

Wang X ，Li Y ，Peng L ，Chen H ，Xia Q ，Zhao P ... -  《-》

GC/MS-based metabolomics analysis reveals active fatty acids biosynthesis in the Filippi's gland of the silkworm, Bombyx mori, during silk spinning.

The Filippi's gland, also called the Lyonet's gland, is in truth a pair of tiny glands that are unique to lepidopteran insects. Although the ultrastructure of the Filippi's gland has been well-understood, the specific biological function of this gland in silk spinning is still unclear. Previous studies proposed a hypothesis that this gland might synthesize and secrete some substances into the anterior silk gland (ASG) to help silk spinning. In order to identify these metabolites, a GC/MS-based metabolomics technique was introduced. A total of 59 metabolites, including fatty acids, amino acids, and sugars, were identified in glands from silkworm larvae in the feeding and silk spinning stages. Abundance and pathway analyses revealed that these metabolites had different abundances during gland development and silk spinning, which may facilitate the transport of small molecules and ions. The most interesting result is that the Filippi's gland has a very active fatty acid biosynthesis process during spinning, suggesting that it may synthesize lipids or waxes and secrete them into the ASG to promote silk spinning. This data provides instructive insight into the biological functions of Filippi's gland from both silkworms and other lepidoptera.

Wang X ，Li Y ，Liu Q ，Tan X ，Xie X ，Xia Q ，Zhao P ... -  《-》

Proteome profile of spinneret from the silkworm, Bombyx mori.

The silkworm spinneret is an important tissue for silk fibrillogenesis and spinning. All biochemical processes during silk fibrillogenesis are correlated with silk properties. Understanding the role of spinneret in silk fibrillogenesis may help to reveal the mechanism of silk fibrillogenesis as well as improve silk quality for commercial purposes. Thus, we profiled the proteome of silkworm spinneret. A total of 1572 proteins and 232 differential abundance proteins were identified. Silk fibrillogenesis-related proteins, such as cuticle proteins, ion-transporting proteins, muscular proteins, and energy metabolic proteins, were abundant in spinneret. Metabolic pathway and GO enrichment analyses revealed that the identified proteins were involved in energy metabolism, chitin binding, and cuticle construction. Active energy metabolism may provide abundant energy for the muscle contraction as well as ion and water exchange. The chitin binding and cuticle construction process may provide sufficient shear forces for silk formation. Our data suggest that silkworm spinneret provides a suitable physiological and biochemical environment for silk fibrillogenesis. These proteins are potential targets for improving silk quality in the silk industry. Data are available via ProteomeXchange with identifier PXD004455.

Wang X ，Li Y ，Liu Q ，Xia Q ，Zhao P ... -  《-》

Comparative transcriptome analysis of silkworm, Bombyx mori colleterial gland suggests their functional role in mucous secretion.

Yang L ，Gao Q ，Dai J ，Yuan G ，Wang L ，Qian C ，Zhu B ，Liu C ，Wei G ... -  《-》

Comparative analysis of the silk gland transcriptomes between the domestic and wild silkworms.

Fang SM ，Hu BL ，Zhou QZ ，Yu QY ，Zhang Z ... -  《BMC GENOMICS》