Effects of Autumn and Spring Heat Waves on Seed Germination of High Mountain Plants.

Alpine plants are considered to be particularly vulnerable to climate change and related extreme episodes, such as heat waves. Despite growing interest in the impact of heat waves on alpine plants, knowledge about their effects on regeneration is still fragmentary. Recruitment from seeds will be crucial for the successful migration and survival of these species and will play a key role in their future adaptation to climate change. In this study, we assessed the impacts of heat waves on the seed germination of 53 high mountain plants from the Northern Apennines (Italy). The seeds were exposed to laboratory simulations of three seasonal temperature treatments, derived from real data recorded at a meteorological station near the species growing site, which included two heat wave episodes that occurred both in spring 2003 and in autumn 2011. Moreover, to consider the effect of increasing drought conditions related to heat waves, seed germination was also investigated under four different water potentials. In the absence of heat waves, seed germination mainly occurred in spring, after seeds had experienced autumn and winter seasons. However, heat waves resulted in a significant increase of spring germination in c. 30% of the species and elicited autumn germination in 50%. When heat waves were coupled with drought, seed germination decreased in all species, but did not stop completely. Our results suggest that in the future, heat waves will affect the germination phenology of alpine plants, especially conditionally dormant and strictly cold-adapted chorotypes, by shifting the emergence time from spring to autumn and by increasing the proportion of emerged seedlings. The detrimental effects of heat waves on recruitment success is less likely to be due to the inhibition of seed germination per se, but rather due to seedling survival in seasons, and temperature and water conditions that they are not used to experiencing. Changes in the proportion and timing of emergence suggest that there may be major implications for future plant population size and structure.

Orsenigo S ，Abeli T ，Rossi G ，Bonasoni P ，Pasquaretta C ，Gandini M ，Mondoni A ... -  《PLoS One》

Climate warming could shift the timing of seed germination in alpine plants.

Despite the considerable number of studies on the impacts of climate change on alpine plants, there have been few attempts to investigate its effect on regeneration. Recruitment from seeds is a key event in the life-history of plants, affecting their spread and evolution and seasonal changes in climate will inevitably affect recruitment success. Here, an investigation was made of how climate change will affect the timing and the level of germination in eight alpine species of the glacier foreland. Using a novel approach which considered the altitudinal variation of temperature as a surrogate for future climate scenarios, seeds were exposed to 12 different cycles of simulated seasonal temperatures in the laboratory, derived from measurements at the soil surface at the study site. Under present climatic conditions, germination occurred in spring, in all but one species, after seeds had experienced autumn and winter seasons. However, autumn warming resulted in a significant increase in germination in all but two species. In contrast, seed germination was less sensitive to changes in spring and/or winter temperatures, which affected only three species. Climate warming will lead to a shift from spring to autumn emergence but the extent of this change across species will be driven by seed dormancy status. Ungerminated seeds at the end of autumn will be exposed to shorter winter seasons and lower spring temperatures in a future, warmer climate, but these changes will only have a minor impact on germination. The extent to which climate change will be detrimental to regeneration from seed is less likely to be due to a significant negative effect on germination per se, but rather to seedling emergence in seasons that the species are not adapted to experience. Emergence in autumn could have major implications for species currently adapted to emerge in spring.

Mondoni A ，Rossi G ，Orsenigo S ，Probert RJ ... -  《-》

Germination responses to winter warm spells and warming vary widely among woody plants in a temperate forest.

Winter underpins key ecological processes, such as dormancy loss and seedling emergence. Enhanced warm spells, together with warming are occurring and will continue in the future. The consequences of these climate phenomena on germination were investigated among co-occurring woody plants, whose seeds are bird-dispersed in autumn and require cold stratification for spring emergence. Seeds from nine common southeastern USA plants were collected in autumn. We verified that seeds of the study species required cold stratification for dormancy loss. We then examined the following aspects in the laboratory or field: effect of warm spells during cold stratification on germination, effect of a warm spell during winter on seed survival and germination phenology, and effect of warming from autumn dispersal through winter dormancy loss on timing of germination. While no consistent effects of warm spells were found in the laboratory on quantity of germination, warm spells advanced spring field germination for several species. Some species germinated during cold stratification and during warm spells, especially extreme spells, in the laboratory. In the field, about half of Lonicera maackii seedlings that emerged with a warm spell died by late winter. With warming from autumn through spring, laboratory germination shifted from spring to predominately autumn for some species. With precocious germination during warm spells or germination phenology shifts, two scenarios are possible. Seedlings may die during winter, reducing the size of the soil seed bank and number of emergents, or they would survive in warmer winters, which would give them a competitive advantage over spring-emerging seedlings.

Flanigan NP ，Bandara R ，Wang F ，Jastrzębowski S ，Hidayati SN ，Walck JL ... -  《-》

Climate warming could increase recruitment success in glacier foreland plants.

Glacier foreland plants are highly threatened by global warming. Regeneration from seeds on deglaciated terrain will be crucial for successful migration and survival of these species, and hence a better understanding of the impacts of climate change on seedling recruitment is urgently needed to predict future plant persistence in these environments. This study presents the first field evidence of the impact of climate change on recruitment success of glacier foreland plants. Seeds of eight foreland species were sown on a foreland site at 2500 m a.s.l., and at a site 400 m lower in altitude to simulate a 2·7 °C increase in mean annual temperature. Soil from the site of origin was used to reproduce the natural germination substrate. Recruitment success, temperature and water potential were monitored for 2 years. The response of seed germination to warming was further investigated in the laboratory. At the glacier foreland site, seedling emergence was low (0 to approx. 40 %) and occurred in summer in all species after seeds had experienced autumn and winter seasons. However, at the warmer site there was a shift from summer to autumn emergence in two species and a significant increase of summer emergence (13-35 % higher) in all species except two. Survival and establishment was possible for 60-75 % of autumn-emerged seedlings and was generally greater under warmer conditions. Early snowmelt in spring caused the main ecological factors enhancing the recruitment success. The results suggest that warming will influence the recruitment of glacier foreland species primarily via the extension of the snow-free period in spring, which increases seedling establishment and results in a greater resistance to summer drought and winter extremes. The changes in recruitment success observed here imply that range shifts or changes in abundance are possible in a future warmer climate, but overall success may be dependent on interactions with shifts in other components of the plant community.

Mondoni A ，Pedrini S ，Bernareggi G ，Rossi G ，Abeli T ，Probert RJ ，Ghitti M ，Bonomi C ，Orsenigo S ... -  《-》

Seed dormancy and germination changes of snowbed species under climate warming: the role of pre- and post-dispersal temperatures.

Climate warming has major impacts on seed germination of several alpine species, hence on their regeneration capacity. Most studies have investigated the effects of warming after seed dispersal, and little is known about the effects a warmer parental environment may have on germination and dormancy of the seed progeny. Nevertheless, temperatures during seed development and maturation could alter the state of dormancy, affecting the timing of emergence and seedling survival. Here, the interplay between pre- and post-dispersal temperatures driving seed dormancy release and germination requirements of alpine plants were investigated. Three plant species inhabiting alpine snowbeds were exposed to an artificial warming treatment (i.e. +1·5 K) and to natural conditions in the field. Seeds produced were exposed to six different periods of cold stratification (0, 2, 4, 8, 12 and 20 weeks at 0 °C), followed by four incubation temperatures (5, 10, 15 and 20 °C) for germination testing. A warmer parental environment produced either no or a significant increase in germination, depending on the duration of cold stratification, incubation temperatures and their interaction. In contrast, the speed of germination was less sensitive to changes in the parental environment. Moreover, the effects of warming appeared to be linked to the level of (physiological) seed dormancy, with deeper dormant species showing major changes in response to incubation temperatures and less dormant species in response to cold stratification periods. Plants developed under warmer climates will produce seeds with changed germination responses to temperature and/or cold stratification, but the extent of these changes across species could be driven by seed dormancy traits. Transgenerational plastic adjustments of seed germination and dormancy shown here may result from increased seed viability, reduced primary and secondary dormancy state, or both, and may play a crucial role in future plant adaptation to climate change.

Bernareggi G ，Carbognani M ，Mondoni A ，Petraglia A ... -  《-》