Acute and sub-acute toxicity of a lyophilised aqueous extract of the aerial part of Spilanthes africana Delile in rats.

Spilanthes africana is a plant used in several countries for the treatment of toothache, malaria, fracture, pneumonia, and dysentery. In order to establish the safety of aerial part of the plant extract, the acute and sub-acute toxicity of the aqueous extract of this plant has been evaluated in male and female young rats. In acute toxicity, the effects of a single oral dose (2,000 mg/kg and 5,000 mg/kg) of the lyophilised aqueous extract have been determined. General behaviour, adverse effects and mortality were determined for up to 14 days. In sub-acute treatment, the effects of the extract in daily single oral administration at the doses of 250, 500 and 1,000 mg/kg during 28 days were evaluated. One group treated at the dose of 1,000 mg/kg for 28 days was let without treatment during 14 days to assess the possible reversibility of the harmful effects of the extract. Body weight, food and water intakes, biochemical and haematological parameters were recorded. Histopathological examination of liver, kidney and lungs were assessed. In acute study, a single administration of the aqueous extract at the doses of 2,000 mg/kg or 5,000 mg/kg did not induce mortality. Thus, the LD50 of the aqueous extract of S. africana has been estimated higher than 5,000 mg/kg. Four hours after administration of the extract, a reduction of the mobility, sensitivity to the noise and to touch has been observed. In sub-acute study, the administration of the extract during 28 days at all doses did not significantly modify the body weight. On the haematological analysis, a decrease of the rate of monocytes and a rise of lymphocytes counts were observed among the male group. In both sexes, it appeared a decrease of the rate of granulocytes two weeks after stopping the treatment. It has also been observed in different groups among the females, an increase of the mean corpuscular content and the mean concentration in haemoglobin as well as an increase of platelets. A significant decrease of transaminases, alkaline phosphatase, triglycerides, and a significant increase of total bilirubin compared to the normal group has been observed. There was a significant decrease in renal catalase in both sexes compared with different control groups. Besides, a significant increase of the kidney rates of glutathione and malondialdehyde have also been observed in the female treated at the doses of 1,000 mg/kg. Histopathological analysis has shown vascular congestion and leucocyte infiltrations in the liver of animals treated at the dose of 1,000 mg/kg. This congestion has been marked in satellite group. In the kidney female satellite group, tubular clarifications have been observed and disappear when stopping the treatment. These results show that the aqueous extract of S. africana given by the oral route is slightly toxic. However in sub-acute treatment, higher doses could provoke functional and structural changes in the organism which could in part reversible. Thus the extract should be used with caution.

Ngueguim TF ，Djouwoug Noussi C ，Donfack JH ，Gounoue KR ，Mbatchou A ，Kamtchouing P ，Dimo T ... -  《-》

Acute and subacute toxicity of aqueous extract of the tuber of Kedrostis africana (L.) Cogn in Wistar rats.

Unuofin JO ，Otunola GA ，Afolayan AJ 《-》

Acute and sub-acute toxicity of the methanolic extract of Pteleopsis hylodendron stem bark.

Pteleopsis hylodendron is one of the most popular medicinal plants in Cameroon where it is used to treat measles, chickenpox, sexually transmitted diseases, female sterility, liver and kidney disorders as well as dropsy. To date there is no documented evidence corroborating its safety. This study thus aimed to determine the toxicity profile of the methanolic extract of Pteleopsis hylodendron. The acute and sub-chronic toxicity of the methanolic extract of Pteleopsis hylodendron were investigated by employing established methods. The acute toxicity study was done by administering single doses (2-8 g/kg body weight) of plant extract to adult mice. For the sub chronic toxicity study, doses (85-680 mg/kg bw) of plant extract were administered daily to adult rats during 28 days after which the effect on organs, the hematological and biochemical parameters was assessed. In mice, single oral administrations of the methanolic extract of Pteleopsis hylodendron caused dose-dependent general behaviour adverse effects and mortality. The LD50 values were 3.00 and 3.60 g/kg bw for males and females respectively. In rats, daily single oral doses of the methanolic extract of Pteleopsis hylodendron provoked significant (p < 0.05) growth retardation in rats at all tested doses after 28 days of dosing. Haematological parameters showed a significant decrease in white blood cells count and significant increases red blood cells count; irrespective of the sex, all biochemical parameters studied, except triglycerides significantly (p < 0.001) increased with dose. However, a dose-dependent significant (p < 0.007) increase in HDL was observed only in male rats. Increases in liver enzymes (ALT and AST), proteins and creatinine levels correlate the observed histopathological damages (i.e. inflammation and vascular congestions) in the liver and kidneys. The overall results of this study indicate that the methanolic extract of Pteleopsis hylodendron stem bark possesses hepatotoxic and nephrotoxic effects at doses ≥ 85 mg/kg bw, suggesting that this plant should be used with caution.

Nana HM ，Ngane RA ，Kuiate JR ，Mogtomo LM ，Tamokou JD ，Ndifor F ，Mouokeu RS ，Etame RM ，Biyiti L ，Zollo PH ... -  《-》

Acute and sub-chronic toxicity of an aqueous extract of the leaves of Herniaria glabra in rodents.

Rhiouani H ，El-Hilaly J ，Israili ZH ，Lyoussi B ... -  《JOURNAL OF ETHNOPHARMACOLOGY》

Acute and sub-chronic oral toxicity profiles of the aqueous extract of Cortex Dictamni in mice and rats.

Cortex Dictamni is used in Chinese folk medicine for the treatment of jaundice, cough, rheumatism and some skin diseases; however, its possible toxicity has not been rigorously studied. The present investigation was carried out to evaluate the safety of Cortex Dictamni aqueous extract (CDAE) by acute and sub-chronic toxicity studies. In acute toxicity tests, seven groups of mice (n=5/group/sex) were orally treated with doses of 0, 28.7, 33.6, 39.7, 46.7, 54.9 and 64.6g/kg of CDAE and general behavior, adverse effects and mortality were recorded for up to 14 days. In sub-chronic toxicity assays, animals received CDAE by gavage at the doses of 0, 3.0, 6.0 or 12.0g/kg/day (n=10/group/sex) for 4 weeks and then followed for a 2-week recovery period. The biochemical, hematological and morphological parameters were determined. In adult mice, single oral administrations of CDAE (0-64.6g/kg body weight) induced an increase in the incidence of general behavioral adverse effects. The mortality rate also increased with increasing dosage (LD50=48.2g/kg). In rats, daily single oral doses of CDAE were well tolerated behaviorally after 4 weeks and induced no significant changes in body weights. However, the absolute and relative liver weight at the end of both administration and recovery periods were significantly elevated, although the histological examination of various organs revealed no differences between the control and the treated groups. The hematological and biochemical parameters were significantly changed; lymphocytes, alanine transaminase, alkaline phosphatase levels showed a significant decrease while neutrophilic granulocyte, albumin, total cholesterol, glucose and blood urea nitrogen levels showed a significant increase, suggesting disturbances of hematopoiesis and liver and kidney functions. Overall, the acute toxicity of CDAE was not clearly observed. However, it is possible that CDAE has a selective toxicity considering the changes in some hematological and liver function parameters and the liver-body weight ratios in the sub-chronic oral toxicity study.

Wang L ，Li Z ，Li L ，Li Y ，Yu M ，Zhou Y ，Lv X ，Arai H ，Xu Y ... -  《-》