Identification of Campylobacter jejuni and determination of point mutations associated with macrolide resistance using a multiplex TaqMan MGB real-time PCR.

The aim of the study was to develop a multiplex real-time PCR method to identify Campylobacter jejuni containing mutations commonly associated with macrolide resistance. A multiplex fluorescence real-time PCR assay was developed based on TaqMan minor groove binder (MGB) probes. The VS1-MGB probe was designed based on the VS1 gene and was used to identify Camp. jejuni. The 23S rDNA-MGB probe was designed to distinguish macrolide resistance mutations in 23S rDNA, while 57D-MGB and 74D-MGB were designed to detect resistance mutations in ribosomal protein L4. The specificity and accuracy of our method were identical to the conventional biochemical tests, mapA PCR, minimum inhibitory concentration (MIC) determination and DNA sequencing. The linear detection limit of the method was 0·03 ng genomic DNA and three colony formation unit (CFU) per reaction. In 6 of 18 cases, the nature of Erythromycin resistance could be correctly determined from natural isolates; absence of the tested mutations was demonstrated in the remaining four resistant isolates. A multiplex TaqMan MGB real-time PCR assay with high specificity and accuracy was developed to simultaneously identify Camp. jejuni and detect the gene mutations associated with macrolide resistance. This multiplex method can potentially simplify the identification of Camp. jejuni and determine macrolide resistance due to mutations in 23S rDNA or ribosomal protein L4. This method has a potential for application in different research areas and molecular surveillance.

Hao H ，Liu J ，Kuang X ，Dai M ，Cheng G ，Wang X ，Peng D ，Huang L ，Ahmad I ，Ren N ，Liu Z ，Wang Y ，Yuan Z ... -  《-》

Macrolide resistance in Campylobacter jejuni and Campylobacter coli: molecular mechanism and stability of the resistance phenotype.

Gibreel A ，Kos VN ，Keelan M ，Trieber CA ，Levesque S ，Michaud S ，Taylor DE ... -  《-》

23S rRNA gene mutations contributing to macrolide resistance in Campylobacter jejuni and Campylobacter coli.

Ladely SR ，Meinersmann RJ ，Englen MD ，Fedorka-Cray PJ ，Harrison MA ... -  《-》

Quantification of mutated alleles of 23S rRNA in macrolide-resistant Campylobacter by TaqMan real-time polymerase chain reaction.

Hao H ，Dai M ，Wang Y ，Chen D ，Yuan Z ... -  《-》

Rapid detection of mutations associated with resistance to erythromycin in Campylobacter jejuni/coli by PCR and line probe assay.

Niwa H ，Chuma T ，Okamoto K ，Itoh K ... -  《INTERNATIONAL JOURNAL OF ANTIMICROBIAL AGENTS》