Antidiabetic, antioxidant and anti inflammatory properties of water and n-butanol soluble extracts from Saharian Anvillea radiata in high-fat-diet fed mice.
According to Saharian traditional medicine, Anvillea radiata Coss. & Dur. (Asteraceae) has been valued for treating a variety of ailments such as gastro-intestinal, liver and pulmonary diseases, and has gained awareness for its beneficial effect on postprandial hyperglycemia. However, to best of our knowledge, no detailed study of the antidiabetic curative effects of this plant has been conducted yet.
To determine the hypoglycemic and antidiabetic effect of dietary supplementation with Anvillea radiata extracts on high-fat-diet (HFD)-induced obesity and insulin resistance in C57BL/6J mice in relation with antioxidant, anti-inflammatory, pancreatic beta-cells and skeletal muscle protection, and digestive enzyme inhibiting properties.
Six extracts (water soluble and organic) from aerial parts of the plant were analyzed phytochemically (total phenolic and flavonoid content) and screened for in vitro superoxide (by chemiluminescence) and hydroxyl radical (by electron paramagnetic resonance spin-trapping) scavenging, antioxidant (DPPH, TRAP and ORAC assays), xanthine oxidase, metal chelating, α-amylase and α-glucosidase inhibitory property, and protective effects on copper-induced lipoprotein oxidation. Then selected hydroalcoholic and aqueous extracts were assessed for toxicity in normal human lung fibroblasts and A549 cancer cells using FMCA and MTT assays. Two water-soluble extracts having the best overall properties were assessed for their (i) protective effect at 1-15µg/mL on metabolic activity of rat insulinoma-derived INS-1 cells exposed to hyperglycemic medium, and (ii) acute hypoglycemic effect on 16-weeks HFD-induced diabetic mice. Then diabetic mice were administered HFD supplemented by extracts (up to 150mg/kg/day) for 12 additional weeks using standard diet as control and the antidiabetic drug, metformin (150mg/kg), as positive control. Then the antidiabetic, anti-inflammatory and antioxidant activity of extracts were determined.
Of the highly efficient polyphenolics-enriched hydroalcoholic and ethyl acetate extracts, the lyophilized aqueous (AQL) and butanol extracts were not toxic in cells (≤ 400µg/mL) or when given orally in normal mice (≤ 2000mg/kg), exerted a dose-dependent hypoglycemic action in diabetic mice, which was maximal at the dose of 150mg/kg. Upon administering this dose for 12 weeks, both extracts significantly ameliorated body weight control capacity, recovery of plasma glucose and insulin level, reduced oxidative stress in blood, myocardial and skeletal muscles, and improved hyperlipidemic and inflammatory status. Moreover, diabetes-related complications were optimally ameliorated by oral therapy based on halved doses (75mg/kg) of a mixture of AQL and metformin.
Current investigation supports the traditional medicinal usage of Anvillea radiata and suggests that both readily accessible and low-cost bio-extracts have the potency to develop an antihyperglycemic, antihyperlipidemic and protective agent against beta-cells and muscle dysfunction at doses compatible with the common practices of indigenous people for the management of metabolic disorders.
Kandouli C
，Cassien M
，Mercier A
，Delehedde C
，Ricquebourg E
，Stocker P
，Mekaouche M
，Leulmi Z
，Mechakra A
，Thétiot-Laurent S
，Culcasi M
，Pietri S
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Blighia sapida leaves halt elevated blood glucose, dyslipidemia and oxidative stress in alloxan-induced diabetic rats.
Blighia sapida leaves are used in the management of diabetes in Nigeria. Thus the antidiabetic activity of methanolic Blighia sapida leaf extract and its capability to halt oxidative stress and dyslipidemia in alloxan-induced diabetic rats were investigated.
In vitro antioxidant activity of the extract (0.2-1.0mg/mL) was investigated using 2,2-diphenyl-1-1picrylhydrazyl (DPPH) radical, superoxide ion, hydrogen peroxide, and hydroxyl radical and ferric ion reducing system. Antidiabetic was evaluated in alloxan-induced diabetic rats.
The methanolic extract of Blighia sapida leaves at 1.0mg/mL scavenged DPPH, superoxide ion, hydrogen peroxide, and hydroxyl radical at 80.34%, 57.39%, 72.36% and 77.0% respectively, while ferric ion was significantly reduced. Single oral dose of the extract significantly reduced blood glucose level in a dose dependent manner with highest dose producing 18.6% reduction after 240min. Similar reduction was produced after 28 days of extract administration with the highest dose producing 65.65% reduction which compared significantly (P<0.05) with the control group and glibenclamide treated groups. Alloxan-induced diabetic mediated alterations in liver and serum cholesterol, triacylglycerides, high-density lipoprotein cholesterol (HDLc), low density lipoprotein (LDL) cholesterol and very low-density lipoprotein cholesterol (VLDLc) were significantly (P<0.05) restored by the extract. Methanolic extract of Blighia sapida leaves significantly attenuated the decrease in the activities of reactive oxygen species detoxifying enzymes (superoxide dismutase, catalase, glutathione peroxidase, glutathione reductase and glucose 6-phosphate dehydrogenase) in the liver and pancreas of alloxan-induced diabetic rats. Elevation in the concentrations of malondialdehyde, conjugated dienes, lipid hydroperoxides, protein carbonyl, and fragmented DNA was significantly (P<0.05) lowered by Blighia sapida leaves extract.
Overall, methanolic extract of Blighia sapida leaves at all doses used reduced blood glucose level and prevented oxidative stress and dyslipidemia in alloxan-induced diabetic rats.
Oloyede OB
，Ajiboye TO
，Abdussalam AF
，Adeleye AO
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