Oxidative stress and innate immunity responses in cigarette smoke stimulated nasal epithelial cells.

Cigarette smoke extracts (CSE) may play a significant role in diseases of the upper airway including chronic rhinosinusitis. Even short term exposure of cigarette smoke has adverse effects on mitochondrial functions and redox homeostasis in tissues which may progress to further complications associated with chronic smoking. Cigarette smoke alters toll-like receptor 4 (TLR4) expression and activation in bronchial epithelial cells. Carbocysteine is an anti-oxidant and mucolytic agent. The effects of carbocysteine on CSE induced oxidative stress and on associated innate immune and inflammatory responses in nasal epithelial cells are largely unknown. The present study was aimed to assess in CSE stimulated nasal epithelial cells (RPMI 2650) the effects of carbocysteine (10(-4)M) on: cell survival, intracellular reactive oxygen species (ROS) production, TLR4 expression, LPS binding and neutrophil chemotaxis (actin reorganization). We found that CSE increased ROS production, TLR4 expression, LPS binding and neutrophil chemotaxis and all these events were counteracted by pre-incubating CSE stimulated RPMI 2650 cells with carbocysteine. In conclusion, the present study provides compelling evidence that carbocysteine may be considered a promising therapeutic strategy in chronic inflammatory nasal diseases.

Pace E ，Ferraro M ，Di Vincenzo S ，Gerbino S ，Bruno A ，Lanata L ，Gjomarkaj M ... -  《-》

The effect of tobacco ingredients on smoke chemistry. Part I: Flavourings and additives.

Baker RR ，Pereira da Silva JR ，Smith G 《FOOD AND CHEMICAL TOXICOLOGY》

Double impact of cigarette smoke and mechanical ventilation on the alveolar epithelial type II cell.

Hirsch J ，Chalkley RJ ，Bentley T ，Burlingame AL ，Frank JA ... -  《-》

Cigarette smoke effects on innate immune mechanisms in the nasal mucosa. Potential effects on the microbiome.

Jaspers I 《-》

Alterations of neutrophil f-actin kinetics by tobacco smoke: implications for periodontal diseases.

Ryder MI ，Wu TC ，Kallaos SS ，Hyun W ... -  《JOURNAL OF PERIODONTAL RESEARCH》