Identification of predictive biomarkers for early diagnosis of larynx carcinoma based on microRNA expression data.

The abnormal expression of microRNAs (miRNAs) plays a key role in tumorigenesis. In order to identify potential miRNA biomarkers for early diagnosis of larynx carcinoma, we employed a miRNA microarray technique and applied bioinformatic algorithms to characterize miRNA classifiers in early larynx carcinoma and normal esophageal mucosa tissue samples from 69 patients who were selected retrospectively for this study. We identified 47 miRNAs that were significantly differentially expressed in primary larynx tumor tissues compared to normal tissues using a SAM algorithm. Of these, 30 were up-regulated and 17 down-regulated in early larynx cancer, including hsa-miR-657, which was overexpressed, and hsa-miR-1287, which was underexpressed. These two candidate miRNA biomarkers were combined as a single classifier to recognize the biological characteristics in early larynx carcinoma. Real-time quantitative reverse-transcription PCR validated the microarray results in both trial and test samples. The hsa-miR-657-hsa-miR-1287 classifier displayed high sensitivity and specificity for discriminating between early larynx carcinoma and normal mucosa tissues, suggesting they may be suitable as potential predictive biomarkers for the early diagnosis of larynx carcinoma.

Wang Y ，Chen M ，Tao Z ，Hua Q ，Chen S ，Xiao B ... -  《Cancer Genetics》

Differential expression profiles of microRNAs as potential biomarkers for the early diagnosis of esophageal squamous cell carcinoma.

Yang M ，Liu R ，Sheng J ，Liao J ，Wang Y ，Pan E ，Guo W ，Pu Y ，Yin L ... -  《-》

Identification of microRNAs differentially expressed between lung squamous cell carcinoma and lung adenocarcinoma.

Hamamoto J ，Soejima K ，Yoda S ，Naoki K ，Nakayama S ，Satomi R ，Terai H ，Ikemura S ，Sato T ，Yasuda H ，Hayashi Y ，Sakamoto M ，Takebayashi T ，Betsuyaku T ... -  《-》

Diagnostic microRNA markers to screen for sporadic human colon cancer in stool: I. Proof of principle.

Ahmed FE ，Ahmed NC ，Vos PW ，Bonnerup C ，Atkins JN ，Casey M ，Nuovo GJ ，Naziri W ，Wiley JE ，Mota H ，Allison RR ... -  《-》

MiR-142-3p as a potential prognostic biomarker for esophageal squamous cell carcinoma.

microRNAs (miRNAs), small non-coding RNAs, are always aberrantly expressed in many diseases including human cancers. The aim of this study was to examine and determine the clinical significance of hsa-miR-31, hsa-miR-142-3p, hsa-miR-338-3p, and hsa-miR-1261 expression in esophageal squamous cell carcinoma (ESCC). Expression levels of four selected miRNAs, initially evaluated by microarray, were validated by qRT-PCR. Various statistical methods were used to analyze the relationship between miRNA expression and clinicopathologic features and prognosis in 91 patients with ESCC. MiR-31 and miR-142-3p expression were correlated to histological differentiation in ESCC (P < 0.05, Student's t-test); high miR-142-3p expression was associated with a poor prognosis in all 91 ESCC patients (P = 0.014, log-rank) and identified as an independent prognostic factor in ESCC (P = 0.017, univariate Cox; P = 0.022, multivariate Cox). More importantly, stratified analysis indicated that high miR-142-3p expression was correlated to a poor prognosis within good-prognosis groups comprised of ESCC patients with small tumor size, negative lymph node metastasis, or early stage (all P < 0.05). The main findings suggest that miR-142-3p is involved in the progression of ESCC and is a potential prognostic biomarker for ESCC.

Lin RJ ，Xiao DW ，Liao LD ，Chen T ，Xie ZF ，Huang WZ ，Wang WS ，Jiang TF ，Wu BL ，Li EM ，Xu LY ... -  《-》