Diagnostic performance of urinary kidney injury molecule-1 and neutrophil gelatinase-associated lipocalin for acute kidney injury in an obstructive nephropathy patient.

We investigated the diagnostic performance of urinary kidney injury molecule-1 (KIM-1) and neutrophil gelatinase-associated lipocalin (NGAL) for acute kidney injury (AKI) in 90 obstructive nephropathy patients. Urine samples were obtained preoperatively and 4, 8, 12, 24, 48 and 72 h postoperatively, and urinary KIM-1 and NGAL contents were measured by enzyme linked immunosorbent assay and corrected against urine creatinine content. The receiver operating characteristic (ROC) curves were used to determine the area under the curve (AUCs) of urinary KIM-1 and NGAL for AKI. The baseline urinary KIM-1 contents were higher in AKI patients than non-AKI patients (P < 0.01). Urinary NGAL contents were also higher in AKI patients than non-AKI patients (P < 0.001). The area under the curve (AUC) of urinary KIM-1 was 0.900 (P = 0.004) and at a cutoff of 338.26 pg/mg Cr, the sensitivity was 90% and the specificity was 75%. The AUC of urinary NGAL was 0.900 (P = 0.004) and at a cutoff of 261.76 ng/mg Cr, the sensitivity was 90% and the specificity was 87.5%. The combined AUC of urinary KIM-1 and NGAL was 0.938 (P = 0.002) with a sensitivity of 90% and a specificity of 100%. Cox regression analysis revealed that urinary KIM-1content 72 h after operation correlated with the prognosis of AKI patients (P = 0.009). When kidney viability was stratified by urinary KIM-1 content 72 h postoperatively, Kaplan-Meier analysis showed that patients with a urinary content of KIM-1 < 138.20 pg/mg had a higher kidney viability rate than those with a urinary content of KIM-1 > 138.20 pg/mg. Urinary KIM-1 and NGAL had a good accuracy for detecting AKI. KIM-1 72 h postoperatively can predict the renal outcome of obstructive nephropathy.

Xue W ，Xie Y ，Wang Q ，Xu W ，Mou S ，Ni Z ... -  《-》

Implication of combined urinary biomarkers in early diagnosis of acute kidney injury following percutaneous coronary intervention.

Luo Q ，Zhou F ，Dong H ，Wu L ，Chai L ，Lan K ，Wu M ... -  《CLINICAL NEPHROLOGY》

[Predict value of monitoring changes of urinary neutrophil gelatinase-associated lipocalin and kidney injury molecule-1 after coronary angiography and percutaneous coronary intervention on early diagnosis of contrast-induced nephropathy].

Wang L ，Pu X 《-》

Clinical implication of urinary neutrophil gelatinase-associated lipocalin and kidney injury molecule-1 in patients with acute paraquat intoxication.

Gil HW ，Yang JO ，Lee EY ，Hong SY ... -  《-》

Effect of long-term storage of urine samples on measurement of kidney injury molecule 1 (KIM-1) and neutrophil gelatinase-associated lipocalin (NGAL).

Levels of urinary biomarkers, such as kidney injury molecule 1 (KIM-1) and neutrophil gelatinase-associated lipocalin (NGAL), can determine the risk of renal damage and predict the outcome of multiple kidney diseases, including transplant rejection. Studies of urinary biomarkers frequently use urine that has been stored frozen before analysis without taking into account the potential of biomarker degradation during storage. Observational study. 39 kidney transplant patients visiting an outpatient clinic. Urine storage conditions (4°C and -80°C with or without protease inhibitors) and centrifugation after thawing of samples. Urinary concentrations of NGAL and KIM-1. NGAL and KIM-1 were measured with commercially available enzyme-linked immunosorbent assay kits. Urinary NGAL and KIM-1 concentrations were stable in urine up to 48 hours when stored at 4°C and up to 6 months when stored at -80°C, independent of the addition of protease inhibitors. A centrifugation step prior to biomarker measurement did not change urinary biomarker concentrations of urine stored at -80°C. Only KIM-1 and NGAL were measured, which possibly limits the relevance of the findings when making determinations about other urinary biomarkers. This study recommends that urine samples collected for the assessment of KIM-1 or NGAL are immediately cooled to 4°C and subsequently frozen at -80°C within 2 days. At -80°C, both KIM-1 and NGAL concentrations are stable for at least 6 months.

van de Vrie M ，Deegens JK ，van der Vlag J ，Hilbrands LB ... -  《-》