The Escherichia coli phylogenetic group B2 with integrons prevails in childhood recurrent urinary tract infections.

The aim of our study was to characterize the phylogenetic groups of Escherichia coli, antibiotic resistance, and containment of class 1 integrons in the first attack of pyelonephritis and in subsequent recurrences in young children. Altogether, 89 urine E. coli isolates from 41 children with urinary tract infection (UTI) were studied for prevalence and persistence of phylogenetic groups by pulsed-field gel electrophoresis (PFGE), antibacterial resistance by minimal inhibitory concentrations (MIC) and class 1 integrons by PCR. Phylogenetic group B2 was most common (57%), followed by D (20%), A (18%) and B1 (5%). Overall resistance to betalactams was 61%, trimethoprim-sulfamethoxazole 28%, and was not associated with phylogenetic groups. According to PFGE, the same clonal strain persisted in 77% of patients. The persistence was detected most often in phylogenetic group B2 (70%). Phylogenetic group B2 more often contained class 1 integrons than group A. Integron positive strains had higher MIC values of cefuroxime, cefotaxime, and gentamicin. In conclusion, phylogenetic group B2 was the most common cause of the first episode of pyelonephritis, as well as in case of the persistence of the same strain and contained frequently class 1 integrons in childhood recurrent UTI. An overall frequent betalactam resistance was equally distributed among phylogenetic groups.

Kõljalg S ，Truusalu K ，Stsepetova J ，Pai K ，Vainumäe I ，Sepp E ，Mikelsaar M ... -  《-》

Bacterial characteristics of importance for recurrent urinary tract infections caused by Escherichia coli.

Ejrnæs K 《-》

Distribution of integron-associated trimethoprim-sulfamethoxazole resistance determinants among Escherichia coli from humans and food-producing animals.

Ho PL ，Wong RC ，Chow KH ，Que TL ... -  《-》

Persistence of Escherichia coli clones and phenotypic and genotypic antibiotic resistance in recurrent urinary tract infections in childhood.

Kõljalg S ，Truusalu K ，Vainumäe I ，Stsepetova J ，Sepp E ，Mikelsaar M ... -  《-》

Integrons and antibiotic resistance in phylogenetic group B2 Escherichia coli.

Skurnik D ，Lacheeb S ，Bernede C ，le Menac'h A ，Elbaz S ，Mohler J ，Denamur E ，Andremont A ，Ruimy R ... -  《-》