The efficiency of combined CaO/electrochemical treatment in removal of acid mine drainage induced toxicity and genotoxicity.

Acid mine drainage (AMD) is a by-product of the mining industry that has a detrimental effect on aquatic plant and animal life due to high load of heavy metals and sulfates. In the present study, the toxic and genotoxic potential of AMD prior to and following combination of neutralization/electrocoagulation processes was evaluated using several bioassays and selected parameters. Regardless of pH correction of AMD prior to Daphnia bioassay, high acute toxicity was observed in Daphnia magna. The mine leachate also induced strong phyto-, cyto- and genotoxicity to Allium cepa roots. Short term exposure to AMD inhibited duckweed growth and chlorophyll a content and simultaneously promoted lipid peroxidation and DNA damage despite duckweed capability to upregulate antioxidative defense mechanisms. The results show that observed (geno)toxicity could be related to oxidative stress most probably induced by toxic metal action. However, influence of low pH as a contributing factor in the phytotoxicity of AMD cannot be excluded. The application of combined treatment eliminated genotoxicity and was highly efficient in reducing toxicity of AMD. Thus, the method seems to be suitable for treatment of AMD waters enabling their safe discharge to an aquatic environment.

Radić S ，Vujčić V ，Cvetković Ž ，Cvjetko P ，Oreščanin V ... -  《-》

Evaluation of the toxic and genotoxic potential of acid mine drainage using physicochemical parameters and bioassays.

Carboniferous activity generates acid mine drainage (AMD) which is capable of unleashing toxic effects on the exposed biota. The aim of this study was to evaluate the toxic and genotoxic potential of untreated-AMD and AMD treated with calcinated sediment, using physicochemical parameters and bioassays. Results revealed that untreated-AMD presented low pH values and elevated concentrations of the metals Fe, Al, Mn, Zn and Cu. High acute toxicity was observed in Artemia sp. and Daphnia magna, and sub-chronic toxicity and genotoxicity in Allium cepa L. as well as scission of plasmid DNA exposed to untreated-AMD. Treatment of AMD with calcinated sediment promoted the reduction of acidity and the removal of metals, as well as a reduction in toxic and genotoxic effects. In conclusion, the calcinated sediment can be used as an alternative AMD treatment.

Netto E ，Madeira RA ，Silveira FZ ，Fiori MA ，Angioleto E ，Pich CT ，Geremias R ... -  《-》

A combined CaO/electrochemical treatment of the acid mine drainage from the "Robule" Lake.

Orescanin V ，Kollar R 《-》

Removal of landfill leachate toxicity and genotoxicity by two treatment methods.

Brkanac SR ，Vujčić V ，Cvjetko P ，Baković V ，Oreščanin V ... -  《-》

Use of coal mining waste for the removal of acidity and metal ions Al (III), Fe (III) and Mn (II) in acid mine drainage.

Geremias R ，Laus R ，Macan JM ，Pedrosa RC ，Laranjeira MC ，Silvano J ，Fávere FV ... -  《ENVIRONMENTAL TECHNOLOGY》