Aberrant histone modification in peripheral blood B cells from patients with systemic sclerosis.

To investigate alterations in histone modifications in B cells and their role in the pathogenesis of systemic sclerosis (SSc). Global histone H3/H4 acetylation and H3K4/H3K9 methylation in B cells of SSc were tested by EpiQuik™ assay kits. Related histone modifier enzymes were measured by RT-PCR and Western blot. Global histone H4 hyperacetylation and global histone H3K9 hypomethylation were observed in SSc B cells compared with controls. Expression of JHDM2A was significantly increased but HDAC2, HDAC7, and, SUV39H2 were significantly down-regulated in SSc B cells relative to controls. Global histone H4 acetylation and the expression of HDAC2 were negatively correlated. Global histone H3K9 methylation and the expression of SUV39H2 protein were positively correlated. Global H4 acetylation was positively correlated with disease activity and expression of HDAC2 protein was negatively correlated with skin thickness. Histone modifications were altered in B cells in SSc correlating with skin thickness and disease activity.

Wang Y ，Yang Y ，Luo Y ，Yin Y ，Wang Q ，Li Y ，Kanekura T ，Wang J ，Liang G ，Zhao M ，Lu Q ，Xiao R ... -  《-》

Abnormal histone modification patterns in lupus CD4+ T cells.

Hu N ，Qiu X ，Luo Y ，Yuan J ，Li Y ，Lei W ，Zhang G ，Zhou Y ，Su Y ，Lu Q ... -  《JOURNAL OF RHEUMATOLOGY》

Aberrant histone acetylation and methylation levels in woman with endometriosis.

Xiaomeng X ，Ming Z ，Jiezhi M ，Xiaoling F ... -  《-》

Abnormal epigenetic modifications in peripheral blood mononuclear cells from patients with alopecia areata.

Alopecia areata (AA) is a hair loss disease caused by T-cell-mediated autoimmune reactions against anagen-stage hair follicles. Although the exact aetiology is poorly understood, there is evidence to suggest that both genetic and environmental factors are involved in AA pathogenesis. To analyse DNA methylation and histone modification patterns in peripheral blood mononuclear cells (PBMCs) of patients with AA. PBMC samples were obtained from 25 patients with AA and 20 healthy controls. Global DNA methylcytosine levels, as well as histone acetylation and methylation levels, were measured by enzyme-linked immunosorbent assay. mRNA expression levels were determined using real-time quantitative reverse transcription-polymerase chain reaction. Genomic DNA methylation in PBMCs of patients with AA was increased relative to controls. DNMT1, MBD1 and MBD4 expression levels were significantly higher in AA PBMCs than in controls, and DNMT1 transcription levels positively correlated with global DNA methylation levels in patient samples. Histone H3 acetylation was significantly increased and histone H3 lysine 4 methylation was significantly decreased in patient PBMCs compared with healthy controls. Histone H3 acetylation levels were positively correlated with AA disease severity, and with RANTES (CCL5) mRNA expression in PBMCs of patients with AA. These changes were accompanied by increased p300 (EP300), histone deacetylase 1 (HDAC1), myeloid/lymphoid or mixed lineage leukemia (MLL), SET7/9 (SETD7), G9A (EHMT2), JMJD2C (KDM4C) and JARID1A (KDM5A) expression, as well as reduced HDAC2, HDAC7, LSD1 (KDM1A), JMJD2A (KDM4A) and JMJD2B (KDM4B) expression. DNA methylation and histone modification status are altered in PBMCs of patients with AA, possibly due to the deregulation of epigenetic regulatory genes. These changes may contribute to the activation of pathological immune responses in AA.

Zhao M ，Liang G ，Wu X ，Wang S ，Zhang P ，Su Y ，Yin H ，Tan Y ，Zhang J ，Lu Q ... -  《-》

Curcumin modulates the effect of histone modification on the expression of chemokines by type II alveolar epithelial cells in a rat COPD model.

Gan L ，Li C ，Wang J ，Guo X ... -  《International Journal of Chronic Obstructive Pulmonary Disease》