Rapid identification of Gram-negative organisms from blood culture bottles using a modified extraction method and MALDI-TOF mass spectrometry.

The application of matrix-assisted laser desorption/ionisation time-of-flight (MALDI-TOF) mass spectrometry (MS) directly to blood culture broth has potential to identify bloodstream infection earlier and facilitate timely management. We prospectively tested a novel, rapid, and inexpensive in-house spin-lysis protocol with formic acid extraction and compared MALDI-TOF MS identification of Gram-negative bacteria with traditional phenotypic methods (Phoenix™) directly from 318 BACTEC™ (Becton Dickinson, Franklin Lakes, USA) blood cultures. The MS score was ≥1.7 in 268 (91.8%) monomicrobial broths, with concordance to genus and species level of 100% and 97.0%, respectively. MALDI-TOF MS still has limited capacity to detect all species in polymicrobial broths.

Gray TJ ，Thomas L ，Olma T ，Iredell JR ，Chen SC ... -  《-》

Direct common gram-negative bacterial identification from positive blood culture bottles by SELDI-TOF MS.

A protein database was constructed and validated with identification rate over 90% for the 4 most common Gram-negative bacteria on agar plates. By protein masses comparison, 120 bacteria of the 4 species from blood culture bottles were identified. The concordance was high (Kappa=0.906) between our method and conventional approach.

Xiao D ，Yang Y ，Jiang W ，Zhang H ，Liu H ，Yu H ，Xie C ，Zhong M ，Chen L ，Huang W ... -  《-》

Evaluation of matrix-assisted laser desorption ionization-time-of-flight mass spectrometry for species identification of nonfermenting Gram-negative bacilli.

Matrix-assisted laser desorption ionization-time-of-flight mass spectrometry (MALDI-TOF MS) to identify 396 Nonfermenting Gram-Negative Bacilli clinical isolates was evaluated in comparison with conventional phenotypic tests and/or molecular methods. MALDI-TOF MS identified to species level 256 isolates and to genus or complex level 112 isolates. It identified 29 genera including uncommon species.

Almuzara M ，Barberis C ，Traglia G ，Famiglietti A ，Ramirez MS ，Vay C ... -  《-》

[Identification of staphylococci directly from positive blood culture bottles by MALDI-TOF MS system].

Kilic A ，Baysallar M 《MIKROBIYOLOJI BULTENI》

Fast and reliable bacterial identification direct from positive blood culture using a new TFA sample preparation protocol and the Vitek® MS system.

A simple and reliable protocol using 0.1% trifluoroacetic acid (TFA) was developed to identify bacteria directly from blood cultures on the Vitek® MS system. Results presented a correlation of 99% for Gram-negative bacteria at species level, and 86.3% and 82.3% of Gram-positive bacteria at the genus and species levels, respectively.

Monteiro J ，Inoue FM ，Lobo AP ，Sugawara EK ，Boaretti FM ，Tufik S ... -  《-》