SLAM family markers resolve functionally distinct subpopulations of hematopoietic stem cells and multipotent progenitors.

Hematopoietic stem cells (HSCs) and multipotent hematopoietic progenitors (MPPs) are routinely isolated using various markers but remain heterogeneous. Here we show that four SLAM family markers, CD150, CD48, CD229, and CD244, can distinguish HSCs and MPPs from restricted progenitors and subdivide them into a hierarchy of functionally distinct subpopulations with stepwise changes in cell-cycle status, self-renewal, and reconstituting potential. CD229 expression largely distinguished lymphoid-biased HSCs from rarely dividing myeloid-biased HSCs, enabling prospective enrichment of these HSC subsets. Differences in CD229 and CD244 expression resolved CD150(-)CD48(-/low)Lineage(-/low)Sca-1(+)c-Kit(+) cells into a hierarchy of highly purified MPPs that retained erythroid and platelet potential but exhibited progressive changes in mitotic activity and reconstituting potential. Use of these markers, and reconstitution assays, showed that conditional deletion of Scf from endothelial cells and perivascular stromal cells eliminated the vast majority of bone marrow HSCs, including nearly all CD229(-/low) HSCs, demonstrating that quiescent HSCs are maintained by a perivascular niche.

Oguro H ，Ding L ，Morrison SJ 《-》

SLAM family receptors distinguish hematopoietic stem and progenitor cells and reveal endothelial niches for stem cells.

Kiel MJ ，Yilmaz OH ，Iwashita T ，Yilmaz OH ，Terhorst C ，Morrison SJ ... -  《Cell》

Enhanced purification of fetal liver hematopoietic stem cells using SLAM family receptors.

Kim I ，He S ，Yilmaz OH ，Kiel MJ ，Morrison SJ ... -  《-》

SLAM family markers are conserved among hematopoietic stem cells from old and reconstituted mice and markedly increase their purity.

Yilmaz OH ，Kiel MJ ，Morrison SJ 《-》

Hierarchically related lineage-restricted fates of multipotent haematopoietic stem cells.

Carrelha J ，Meng Y ，Kettyle LM ，Luis TC ，Norfo R ，Alcolea V ，Boukarabila H ，Grasso F ，Gambardella A ，Grover A ，Högstrand K ，Lord AM ，Sanjuan-Pla A ，Woll PS ，Nerlov C ，Jacobsen SEW ... -  《-》