A novel model of urinary tract differentiation, tissue regeneration, and disease: reprogramming human prostate and bladder cells into induced pluripotent stem cells.

Primary culture and animal and cell-line models of prostate and bladder development have limitations in describing human biology, and novel strategies that describe the full spectrum of differentiation from foetal through to ageing tissue are required. Recent advances in biology demonstrate that direct reprogramming of somatic cells into pluripotent embryonic stem cell (ESC)-like cells is possible. These cells, termed induced pluripotent stem cells (iPSCs), could theoretically generate adult prostate and bladder tissue, providing an alternative strategy to study differentiation. To generate human iPSCs derived from normal, ageing, human prostate (Pro-iPSC), and urinary tract (UT-iPSC) tissue and to assess their capacity for lineage-directed differentiation. Prostate and urinary tract stroma were transduced with POU class 5 homeobox 1 (POU5F1; formerly OCT4), SRY (sex determining region Y)-box 2 (SOX2), Kruppel-like factor 4 (gut) (KLF4), and v-myc myelocytomatosis viral oncogene homolog (avian) (MYC, formerly C-MYC) genes to generate iPSCs. The potential for differentiation into prostate and bladder lineages was compared with classical skin-derived iPSCs. The student t test was used. Successful reprogramming of prostate tissue into Pro-iPSCs and bladder and ureter into UT-iPSCs was demonstrated by characteristic ESC morphology, marker expression, and functional pluripotency in generating all three germ-layer lineages. In contrast to conventional skin-derived iPSCs, Pro-iPSCs showed a vastly increased ability to generate prostate epithelial-specific differentiation, as characterised by androgen receptor and prostate-specific antigen induction. Similarly, UT-iPSCs were shown to be more efficient than skin-derived iPSCs in undergoing bladder differentiation as demonstrated by expression of urothelial-specific markers: uroplakins, claudins, and cytokeratin; and stromal smooth muscle markers: α-smooth-muscle actin, calponin, and desmin. These disparities are likely to represent epigenetic differences between individual iPSC lines and highlight the importance of organ-specific iPSCs for tissue-specific studies. IPSCs provide an exciting new model to characterise mechanisms regulating prostate and bladder differentiation and to develop novel approaches to disease modelling. Regeneration of bladder cells also provides an exceptional opportunity for translational tissue engineering.

Moad M ，Pal D ，Hepburn AC ，Williamson SC ，Wilson L ，Lako M ，Armstrong L ，Hayward SW ，Franco OE ，Cates JM ，Fordham SE ，Przyborski S ，Carr-Wilkinson J ，Robson CN ，Heer R ... -  《-》

Direct Reprogramming of Human Primordial Germ Cells into Induced Pluripotent Stem Cells: Efficient Generation of Genetically Engineered Germ Cells.

Bazley FA ，Liu CF ，Yuan X ，Hao H ，All AH ，De Los Angeles A ，Zambidis ET ，Gearhart JD ，Kerr CL ... -  《-》

Robust and highly efficient hiPSC generation from patient non-mobilized peripheral blood-derived CD34(+) cells using the auto-erasable Sendai virus vector.

Okumura T ，Horie Y ，Lai CY ，Lin HT ，Shoda H ，Natsumoto B ，Fujio K ，Kumaki E ，Okano T ，Ono S ，Tanita K ，Morio T ，Kanegane H ，Hasegawa H ，Mizoguchi F ，Kawahata K ，Kohsaka H ，Moritake H ，Nunoi H ，Waki H ，Tamaru SI ，Sasako T ，Yamauchi T ，Kadowaki T ，Tanaka H ，Kitanaka S ，Nishimura K ，Ohtaka M ，Nakanishi M ，Otsu M ... -  《Stem Cell Research & Therapy》

Inducing Pluripotency in the Domestic Cat (Felis catus).

Dutton LC ，Dudhia J ，Guest DJ ，Connolly DJ ... -  《-》

NKX3-1 is required for induced pluripotent stem cell reprogramming and can replace OCT4 in mouse and human iPSC induction.

Mai T ，Markov GJ ，Brady JJ ，Palla A ，Zeng H ，Sebastiano V ，Blau HM ... -  《-》