Profile of regulatory T cells and interferon γ secretion in the tumor-draining lymph node from mouse Hepa1-6 cells.

The tumor-draining lymph node (TDLN) is the critical and initial site of the immune decision made between activation and tolerance to tumor antigens. Tumor-reactive lymphadenopathy in TDLN has been observed for decades, but the profiles of immune regulation in these nodes remain unclear. Both regulatory T cells (Tregs) and effector T cells were examined using 6 × 10(5) Hepa1-6 hepatocellular carcinoma cells implanted in footpads of syngeneic C57BL/6J mice, which formed TDLN. FOXP3(+) Tregs and CD8(+) T cells in TDLN were detected by immunohistochemical staining. The frequency of CD4(+)FOXP3(+) T cells and FOXP3 mRNA expression were determined by flow cytometry and real-time quantitative polymerase chain reaction. The interferon γ secretion ability of CD8(+) T cells in TDLN was measured by enzyme-linked immunospot technique. There was significant expansion of Tregs and CD8(+) T cells in the tumor-draining popliteal lymph node compared with nondraining popliteal lymph node and spleen in the same mouse. Tregs were diffusely distributed in the CD8(+) T cell compartment. The CD8(+) T cells primed in TDLN showed a strong ability of interferon γ secretion via in vitro stimulation. These findings support the notion that Tregs suppress CD8(+) T cells by secreting cytokines such as transforming growth factor β and interleukin 10, but do not make CD8(+) T cells lose function in the TDLN. Deletion of Tregs at the secondary lymphoid organs could be crucial for the establishment of a tumor-specific immunotherapy.

Wang LK ，Kuang M ，Hua YP ，He Q ，Chen B ，Wang Y ，Peng BG ... -  《-》

Increased regulatory T cells correlate with CD8 T-cell impairment and poor survival in hepatocellular carcinoma patients.

Fu J ，Xu D ，Liu Z ，Shi M ，Zhao P ，Fu B ，Zhang Z ，Yang H ，Zhang H ，Zhou C ，Yao J ，Jin L ，Wang H ，Yang Y ，Fu YX ，Wang FS ... -  《GASTROENTEROLOGY》

[Clinical significance of CD4+ CD25+ regulatory T-cells detection in tumor-draining lymph nodes of nonsmall cell lung cancer patients].

Su YJ ，Ren K ，Li H ，Ren XB ，Wang CL ... -  《-》

Stimulation of α7 nicotinic acetylcholine receptor by nicotine increases suppressive capacity of naturally occurring CD4+CD25+ regulatory T cells in mice in vitro.

Wang DW ，Zhou RB ，Yao YM ，Zhu XM ，Yin YM ，Zhao GJ ，Dong N ，Sheng ZY ... -  《-》

Intratumoral interleukin-21 increases antitumor immunity, tumor-infiltrating CD8+ T-cell density and activity, and enlarges draining lymph nodes.

Søndergaard H ，Galsgaard ED ，Bartholomaeussen M ，Straten PT ，Odum N ，Skak K ... -  《-》