A rice stress-responsive NAC gene enhances tolerance of transgenic wheat to drought and salt stresses.

Drought and salinity are the primary factors limiting wheat production worldwide. It has been shown that a rice stress-responsive transcription factor encoded by the rice NAC1 gene (SNAC1) plays an important role in drought stress tolerance. Therefore, we introduced the SNAC1 gene under the control of a maize ubiquitin promoter into an elite Chinese wheat variety Yangmai12. Plants expressing SNAC1 displayed significantly enhanced tolerance to drought and salinity in multiple generations, and contained higher levels of water and chlorophyll in their leaves, as compared to wild type. In addition, the fresh and dry weights of the roots of these plants were also increased, and the plants had increased sensitivities to abscisic acid (ABA), which inhibited root and shoot growth. Furthermore, quantitative real-time polymerase chain reactions revealed that the expressions of genes involved in abiotic stress/ABA signaling, such as wheat 1-phosphatidylinositol-3-phosphate-5-kinase, sucrose phosphate synthase, type 2C protein phosphatases and regulatory components of ABA receptor, were effectively regulated by the alien SNAC1 gene. These results indicated high and functional expression of the rice SNAC1 gene in wheat. And our study provided a promising approach to improve the tolerances of wheat cultivars to drought and salinity through genetic engineering.

Saad AS ，Li X ，Li HP ，Huang T ，Gao CS ，Guo MW ，Cheng W ，Zhao GY ，Liao YC ... -  《-》

Expression of OsWNK9 in Arabidopsis conferred tolerance to salt and drought stress.

With No Lysine (WNK) kinase belongs to ser/thr protein kinase group in which conserved catalytic lysine (K) residue of subdomain II is shifted to subdomain I. In this study, we cloned full-length coding region of WNK9 from Oryza sativa (OsWNK9) and performed in silico studies to confirm the presence of all kinase signature regulatory elements. The transcript analysis revealed that OsWNK9 was strongly down regulated under salinity, drought and ABA stress in shoots. Constitutive expression of OsWNK9 in Arabidopsis thaliana imparted increased tolerance to salt, drought, and ABA stress. Transgenic lines showed healthy phenotypes such as green leaves, achieved higher fresh weight and longer roots under salt, drought and ABA stress as compared to wild-type (WT). Transgenic plants showed better seed germination, higher chlorophyll retention and less water loss under salt and drought stress compared to WT. Promoter/gene expression studies revealed that OsWNK9 were expressed throughout plant tissues with higher expression in roots. Subcellular localization studies of OsWNK9 showed their presence in the nucleus. The transcript analysis of abiotic stress marker genes and ABA dependent genes showed they were highly expressed in transgenic lines compared to WT in response to salt and drought stress. The endogenous ABA level under salt and drought stress in transgenic lines was higher than WT. The results indicated that OsWNK9 may regulate salt and drought response in ABA dependent manner.

Manuka R ，Saddhe AA ，Kumar K 《-》

The abiotic stress-responsive NAC-type transcription factor SlNAC4 regulates salt and drought tolerance and stress-related genes in tomato (Solanum lycopersicum).

Zhu M ，Chen G ，Zhang J ，Zhang Y ，Xie Q ，Zhao Z ，Pan Y ，Hu Z ... -  《-》

Wheat bHLH-type transcription factor gene TabHLH1 is crucial in mediating osmotic stresses tolerance through modulating largely the ABA-associated pathway.

Yang T ，Yao S ，Hao L ，Zhao Y ，Lu W ，Xiao K ... -  《-》

The ERF transcription factor TaERF3 promotes tolerance to salt and drought stresses in wheat.

Salinity and drought are major limiting factors of wheat (Triticum aestivum) productivity worldwide. Here, we report the function of a wheat ERF transcription factor TaERF3 in salt and drought responses and the underlying mechanism of TaERF3 function. Upon treatment with 250 mM NaCl or 20% polyethylene glycol (PEG), transcript levels of TaERF3 were rapidly induced in wheat. Using wheat cultivar Yangmai 12 as the transformation recipient, four TaERF3-overexpressing transgenic lines were generated and functionally characterized. The seedlings of the TaERF3-overexpressing transgenic lines exhibited significantly enhanced tolerance to both salt and drought stresses as compared to untransformed wheat. In the leaves of TaERF3-overexpressing lines, accumulation levels of both proline and chlorophyll were significantly increased, whereas H₂O₂ content and stomatal conductance were significantly reduced. Conversely, TaERF3-silencing wheat plants that were generated through virus-induced gene silencing method displayed more sensitivity to salt and drought stresses compared with the control plants. Real-time quantitative RT-PCR analyses showed that transcript levels of ten stress-related genes were increased in TaERF3-overexpressing lines, but compromised in TaERF3-silencing wheat plants. Electrophoretic mobility shift assays showed that the TaERF3 protein could interact with the GCC-box cis-element present in the promoters of seven TaERF3-activated stress-related genes. These results indicate that TaERF3 positively regulates wheat adaptation responses to salt and drought stresses through the activation of stress-related genes and that TaERF3 is an attractive engineering target in applied efforts to improve abiotic stress tolerances in wheat and other cereals.

Rong W ，Qi L ，Wang A ，Ye X ，Du L ，Liang H ，Xin Z ，Zhang Z ... -  《-》