Insulin-like growth factor II (IGF-II) and follicle stimulating hormone (FSH) combinations can improve the in vitro development of grown oocytes enclosed in caprine preantral follicles.

Evaluate the possible role of IGF-II alone or in association with FSH on in vitro development of isolated caprine preantral follicles. Preantral follicles (≥150 μm) were isolated from goat ovaries and cultured for 18 days in basic αMEM medium (control) or supplemented with IGF-II alone at 20 or 50 ng/ml, named IGF20 and IGF50, respectively, or in combination with recombinant FSH (FSH, IGF20F or IGF50F). During in vitro culture, the follicles were analyzed by using morphology criteria, antrum formation and growth rate as parameters. After 18 days of follicular culture, oocytes equal to or larger than 110 μm were used for in vitro maturation (IVM). Oocyte viability and meiosis resumption were assessed by fluorescence microscopy after labeling with calcein-AM, ethidium homodimer and Hoechst 33342. The IGF20 treatment was the only treatment capable of maintaining the percentage of morphologically normal follicles from D0 until D6 and from D12 to D18 (p>0.05), while in all other treatments the percentage of morphologically normal follicles decreased progressively during 18 days of in vitro culture (p<0.05). At D18, all treatments with IGF-II or FSH resulted in a significantly higher percentage of normal follicles when compared to αMEM alone. The IGF50F treatment provided a significantly higher early antrum formation rate when compared to αMEM and FSH alone. The addition of IGF-II alone (20 or 50 ng/ml) or in combination with FSH prevented oocyte degeneration after IVM. Moreover, the FSH treatment demonstrated a lower percentage of oocyte degeneration when compared to control (4.35% vs. 26.3%, respectively; p<0.05). Regarding meiosis resumption, the IGF20F treatment was the only treatment that significantly differed from αMEM alone. All treatments except the control (αMEM alone) presented oocytes at metaphase II. IGF-II associated with FSH stimulated in vitro follicular development, oocyte viability and meiotic resumption of caprine oocytes after IVM.

Duarte AB ，Araújo VR ，Chaves RN ，da Silva GM ，Luz VB ，Haag KT ，Magalhães-Padilha DM ，Almeida AP ，Lobo CH ，Campello CC ，de Figueiredo JR ... -  《-》

Dynamic medium containing growth differentiation factor-9 and FSH maintains survival and promotes in vitro growth of caprine preantral follicles after long-term in vitro culture.

Alves AM ，Chaves RN ，Rocha RM ，Lima LF ，Andrade PM ，Lopes CA ，Souza CE ，Moura AA ，Campello CC ，Báo SN ，Smitz J ，Figueiredo JR ... -  《-》

The effects of insulin and follicle-simulating hormone (FSH) during in vitro development of ovarian goat preantral follicles and the relative mRNA expression for insulin and FSH receptors and cytochrome P450 aromatase in cultured follicles.

Chaves RN ，Duarte AB ，Rodrigues GQ ，Celestino JJ ，Silva GM ，Lopes CA ，Almeida AP ，Donato MA ，Peixoto CA ，Moura AA ，Lobo CH ，Locatelli Y ，Mermillod P ，Campello CC ，Figueiredo JR ... -  《-》

Expression of follicle-stimulating hormone receptor (FSHR) in goat ovarian follicles and the impact of sequential culture medium on in vitro development of caprine preantral follicles.

Saraiva MV ，Celestino JJ ，Araújo VR ，Chaves RN ，Almeida AP ，Lima-Verde IB ，Duarte AB ，Silva GM ，Martins FS ，Bruno JB ，Matos MH ，Campello CC ，Silva JR ，Figueiredo JR ... -  《-》

In vitro growth and maturation of isolated caprine preantral follicles: Influence of insulin and FSH concentration, culture dish, coculture, and oocyte size on meiotic resumption.

The aims of this study were: (1) to evaluate the effect of different insulin concentrations, alone or in combination with either a fixed FSH concentration or increasing FSH concentrations on the in vitro culture of isolated caprine preantral follicles and (2) to analyze the efficiency of two IVM media and maturation culture systems (with or without coculture with in vivo grown oocytes) on the meiosis resumption. Secondary follicles were cultured for 18 days in a basic medium supplemented with low- or high-insulin concentration alone or with a fixed FSH concentration or with increasing FSH concentrations. Oocytes grown in vivo or in vitro were matured alone or cocultured. The high-insulin concentration associated with fixed FSH treatment had higher meiotic resumption rate (P < 0.05) and was the only treatment capable of producing oocytes in metaphase II. The rates of germinal vesicle, germinal vesicle breakdown, metaphase I, metaphase II (MII), meiotic resumption, and oocyte diameter were similar between the maturation media. In conclusion, a basic medium supplemented with 10-μg/mL insulin and 100-μg/mL FSH throughout the culture period improved meiotic resumption rate and produced MII oocytes from caprine preantral follicles cultured in vitro. The MII rate was similar between in vivo and in vitro grown oocytes ≥110 μm.

Silva GM ，Brito IR ，Sales AD ，Aguiar FLN ，Duarte ABG ，Araújo VR ，Vieira LA ，Magalhães-Padilha DM ，Lima LF ，Alves BG ，Silveira LBR ，Lo Turco EG ，Rodrigues AP ，Campello CC ，Wheeler MB ，Figueiredo JR ... -  《-》