He-Ne laser pretreatment protects wheat seedlings against cadmium-induced oxidative stress.

The aim of the investigation is to determine the effect of He-Ne laser pretreatment of wheat seeds on the resistance of seedlings to cadmium stress. Changes in physiological, biochemical and molecular characters were measured. Our results showed that 150 μM Cd treatment significantly reduced plant height, root length, shoot fresh weight, shoot dry weight, root fresh weight, root dry weight, ascorbate acid (AsA) and glutathione (GSH) concentration, the activities of superoxide dismutase (SOD), peroxidase (POD), catalase (CAT), ascorbate peroxidase (APX) and gene expression levels of SOD, APX, enhanced the concentration of malondialdehyde (MDA), hydrogen peroxide (H(2)O(2)) and the rate of superoxide radical (O(2)(•-)) generation in the wheat seedlings when compared with the control. However, seeds with He-Ne laser pretreatment 5 min conferred tolerance to cadmium stress in wheat seedlings by decreasing the concentration of MDA and H(2)O(2), the rate of O(2)(•-) generation and increasing the gene expression levels of SOD, POD, APX, the activities of SOD, POD, CAT, APX and AsA and GSH concentration. These results suggest that those changes in MDA, O(2)(•-), H(2)O(2), anti-oxidative enzymes, gene expression level and anti-oxidative compounds are responsible for the increase in cadmium stress resistance observed in the experiments. The results also showed that the laser had a positive physiological effect on the growth of cadmium stressed seedlings. This is the first investigation reporting the use of He-Ne laser pretreatment to enhance of wheat seedlings tolerance to cadmium stress.

Qiu Z ，Li J ，Zhang M ，Bi Z ，Li Z ... -  《-》

Microwave pretreatment can enhance tolerance of wheat seedlings to CdCl2 stress.

In order to determine the role of microwave in cadmium stress tolerance of wheat (Triticum aestivum L.), seeds were exposed to microwave radiation for 0, 5, 10 and 15 s (wavelength 125 mm, power density 126 mW cm(-2), 2450 MHz), and when the seedlings were 7 d old (with one fully expanded leaves), they were treated with 150 μM CdCl(2) solution for 10 d. Changes in a number of physiological and biochemical characteristics were measured and used as indicators of the protective capacity of microwave radiation in this experiment. Our results showed that 150 μM CdCl(2) treatment reduced plant height, root length, dry weight, AsA and GSH concentration and the activities of SOD, POD, CAT and APX, enhanced the concentration of MDA, H(2)O(2) and the production rate of O(2)- when compared with the control. However, seeds with microwave pretreatment 5 or 10 s conferred tolerance to cadmium stress in wheat seedlings by decreasing the concentration of MDA and H(2)O(2), the production rate of O(2)- and increasing the activities of SOD, POD, CAT, APX and AsA and GSH concentration. Therefore, antioxidative enzymes and antioxidative compounds may participate in tolerance of wheat seedlings to cadmium stress. The results also showed that the microwave radiation had a positive physiological effect on the growth and development of cadmium stressed seedlings. This is the first investigation reporting the use of microwave pretreatment to enhance cadmium stress tolerance of wheat.

Qiu Z ，Li J ，Zhang Y ，Bi Z ，Wei H ... -  《-》

He-Ne laser preillumination improves the resistance of tall fescue (Festuca arundinacea Schreb.) seedlings to high saline conditions.

Gao LM ，Li YF ，Han R 《-》

Ultrasonic vibration seeds showed improved resistance to cadmium and lead in wheat seedling.

Chen YP ，Liu Q ，Yue XZ ，Meng ZW ，Liang J ... -  《-》

Effects of CO2 laser pretreatment on drought stress resistance in wheat.

Qiu ZB ，Liu X ，Tian XJ ，Yue M ... -  《JOURNAL OF PHOTOCHEMISTRY AND PHOTOBIOLOGY B-BIOLOGY》