Role of KATP channels and TRPV1 receptors in hydrogen sulfide-enhanced gastric emptying of liquid in awake mice.

Hydrogen sulphide (H(2)S) has shown to relax gastrointestinal muscle. Here in, we evaluated the effects of H(2)S donors on gastric emptying and in pyloric sphincter muscle relaxation, and whether these effects involved K(ATP) channels or TRPV1 receptors. Mice were treated with l-cysteine (alone or with propargylglycine-an inhibitor of H(2)S synthesis), NaHS, Lawesson's reagent (H(2)S donors) or saline. After 30 min, mice were gavaged with a liquid meal containing a nonabsorbable marker and then killed at 10, 20 or 30 min intervals to assess marker recovery from the stomach and intestine. This experiment was repeated in mice pre-treated with K(ATP) channel (glibenclamide) or TRPV1 receptor (capsazepine) antagonists. In addition, pyloric sphincter muscles were mounted in an organ bath, incubated with saline, glibenclamide or capsazepine, and NaHS dose-responses were determined. H(2)S donors and l-cysteine enhanced gastric emptying in a dose-dependent manner; propargylglycine reversed the effect of l-cysteine. Both glibenclamide and capsazepine abolished l-cysteine and H(2)S donors' augmentation of gastric emptying. Dose-dependent inductions of pyloric sphincter relaxation by NaHS were abolished by glibenclamide or capsazepine. These data suggest that H(2)S donors-induced acceleration of gastric emptying and relaxation of pyloric sphincter muscle by K(ATP) channel and TRPV1 receptor activations.

Medeiros JV ，Bezerra VH ，Lucetti LT ，Lima-Júnior RC ，Barbosa AL ，Tavares BM ，Magalhães PJ ，Santos AA ，Cunha FQ ，Soares PM ，Souza MH ... -  《-》

Hydrogen sulfide prevents ethanol-induced gastric damage in mice: role of ATP-sensitive potassium channels and capsaicin-sensitive primary afferent neurons.

Medeiros JV ，Bezerra VH ，Gomes AS ，Barbosa AL ，Lima-Júnior RC ，Soares PM ，Brito GA ，Ribeiro RA ，Cunha FQ ，Souza MH ... -  《-》

Role of hydrogen sulfide production in inhibitory action of L-cysteine on isolated porcine irides.

Ohia SE ，Opere CA ，Monjok EM ，Kouamou G ，Leday AM ，Njie-Mbye YF ... -  《-》

Inhibitory action of hydrogen sulfide on muscarinic receptor-induced contraction of isolated porcine irides.

Monjok EM ，Kulkarni KH ，Kouamou G ，McKoy M ，Opere CA ，Bongmba ON ，Njie YF ，Ohia SE ... -  《-》

Dual effect of exogenous hydrogen sulfide on the spontaneous contraction of gastric smooth muscle in guinea-pig.

Zhao P ，Huang X ，Wang ZY ，Qiu ZX ，Han YF ，Lu HL ，Kim YC ，Xu WX ... -  《-》