Bifidobacterium bifidum NCC 453 promotes tolerance induction in murine models of sublingual immunotherapy.

Enhancing clinical efficacy remains a major goal in allergen-specific immunotherapy. In this study, we tested three strains of bifidobacteria as candidate adjuvants for sublingual allergy vaccines. Probiotic candidates were evaluated in human monocyte-derived dendritic cell (h-DC) maturation and CD4(+) T-cell polarization in vitro models and further tested in murine models of sublingual immunotherapy in BALB/c mice sensitized to either ovalbumin or birch pollen. Bifidobacterium adolescentis, B. bifidum and B. longum induced h-DC maturation and polarized naïve CD4(+) T cells toward interferon-γ and interleukin-10 production. B. bifidum increased CD25(high), Foxp3(+) cells within CD4(+) T lymphocytes and was the most potent inducer of interferon-γ in Th2-skewed peripheral blood mononuclear cells and h-DC T-cell cocultures. It also induced a significant decrease in airway hyperresponsiveness in BALB/c mice sensitized to ovalbumin. Sublingual administration of B. bifidum together with recombinant Bet v 1 enhanced tolerance induction in BALB/c mice sensitized to birch pollen, with a downregulation of both airway hyperresponsiveness, lung inflammation and Bet v 1-specific Th2 responses. Due to its capacity to reorient established Th2 responses toward Th1/regulatory T-cell profiles, B. bifidum represents a valid candidate adjuvant for specific immunotherapy of type I allergies.

Moussu H ，Van Overtvelt L ，Horiot S ，Tourdot S ，Airouche S ，Zuercher A ，Holvoet S ，Prioult G ，Nutten S ，Mercenier A ，Mascarell L ，Moingeon P ... -  《-》

Targeting the allergen to oral dendritic cells with mucoadhesive chitosan particles enhances tolerance induction.

Saint-Lu N ，Tourdot S ，Razafindratsita A ，Mascarell L ，Berjont N ，Chabre H ，Louise A ，Van Overtvelt L ，Moingeon P ... -  《-》

Toll-like receptor 2 agonist Pam3CSK4 enhances the induction of antigen-specific tolerance via the sublingual route.

Lombardi V ，Van Overtvelt L ，Horiot S ，Moussu H ，Chabre H ，Louise A ，Balazuc AM ，Mascarell L ，Moingeon P ... -  《-》

Sublingual immunotherapy induces IL-10-producing T regulatory cells, allergen-specific T-cell tolerance, and immune deviation.

Bohle B ，Kinaciyan T ，Gerstmayr M ，Radakovics A ，Jahn-Schmid B ，Ebner C ... -  《JOURNAL OF ALLERGY AND CLINICAL IMMUNOLOGY》

A synthetic triacylated pseudo-dipeptide molecule promotes Th1/TReg immune responses and enhances tolerance induction via the sublingual route.

Mascarell L ，Van Overtvelt L ，Lombardi V ，Razafindratsita A ，Moussu H ，Horiot S ，Chabre H ，Limal D ，Moutel S ，Bauer J ，Chiavaroli C ，Moingeon P ... -  《VACCINE》