Comparative toxicity of two glyphosate formulations (original formulation of Roundup® and Roundup WeatherMAX®) to six North American larval anurans.

The toxicity of two glyphosate formulations (the original formulation of Roundup® and Roundup WeatherMAX®) to six species of North American larval anurans was evaluated by using 96-h static, nonrenewal aqueous exposures. The 96-h median lethal concentration values (LC50) ranged from 1.80 to 4.22 mg acid equivalent (ae)/L and 1.96 to 3.26 mg ae/L for the original formulation of Roundup and Roundup WeatherMAX, respectively. Judged by LC50 values, four species were more sensitive to Roundup WeatherMAX exposures, and two species were more sensitive to the original formulation. Two of six species, Bufo fowleri (p < 0.05, F = 14.89, degrees of freedom [df] = 1) and Rana clamitans (p < 0.05, F = 18.46, df = 1), had significantly different responses to the two formulations tested. Increased sensitivity to Roundup WeatherMAX likely was due to differences in the surfactants or relative amounts of the surfactants in the two formulations. Potency slopes for exposures of the original formulation ranged from 24.3 to 92.5% mortality/mg ae/L. Thresholds ranged from 1.31 to 3.68 mg ae/L, showing an approximately three times difference in the initiation of response among species tested. For exposures of Roundup WeatherMAX, slopes ranged from 49.3 to 84.2% mortality/mg ae/L. Thresholds ranged from 0.83 to 2.68 mg ae/L. Margins of safety derived from a simulated direct overspray were above 1, except for one species in exposures of Roundup WeatherMAX. Laboratory data based on aqueous exposures are conservative because of the lack of environmental ligands; however, these tests provide information regarding the relative toxicity between these two Roundup formulations.

Fuentes L ，Moore LJ ，Rodgers JH Jr ，Bowerman WW ，Yarrow GK ，Chao WY ... -  《-》

Role of sediments in modifying the toxicity of two Roundup formulations to six species of larval anurans.

Fuentes L ，Moore LJ ，Rodgers JH Jr ，Bowerman WW ，Yarrow GK ，Chao WY ... -  《-》

Relative toxicity of the components of the original formulation of Roundup to five North American anurans.

The responses of five North American frog species that were exposed in an aqueous system to the original formulation of Roundup were compared. Carefully designed and un-confounded laboratory toxicity tests are crucial for accurate assessment of potential risks from the original formulation of Roundup to North American amphibians in aquatic environments. The formulated mixture of this herbicide as well as its components, isopropylamine (IPA) salt of glyphosate and the surfactant MON 0818 (containing polyethoxylated tallowamine (POEA)) were separately tested in 96 h acute toxicity tests with Gosner stage 25 larval anurans. Rana pipiens, R. clamitans, R. catesbeiana, Bufo fowleri, and Hyla chrysoscelis were reared from egg masses and exposed to a series of 11 concentrations of the original formulation of Roundup herbicide, nine concentrations of MON 0818 and three concentrations of IPA salt of glyphosate in static (non-renewal) aqueous laboratory tests. LC50 values are expressed as glyphosate acid equivalents (ae) or as mg/L for MON 0818 concentrations for comparison between the formulation and components. R. pipiens was the most sensitive of five species with 96 h-LC50 values for formulation tests, for the five species, ranging from 1.80 to 4.22 mg ae/L, and MON 0818 exposures with 96 h-LC50 values ranging from 0.68 to 1.32 mg/L. No significant mortality was observed during exposures of 96 h for any of the five species exposed to glyphosate IPA salt at concentrations up to 100 times the predicted environmental concentration (PEC). These results agree with previous studies which have noted that the surfactant MON 0818 containing POEA contributes the majority of the toxicity to the herbicide formulations for fish, aquatic invertebrates, and amphibians. These study results suggest that anurans are among the most sensitive species, and emphasize the importance of testing the herbicide formulation in addition to its separate components to accurately characterize the toxicity and potential risk of the formulation.

Moore LJ ，Fuentes L ，Rodgers JH Jr ，Bowerman WW ，Yarrow GK ，Chao WY ，Bridges WC Jr ... -  《-》

The toxicity of Roundup Original Max to 13 species of larval amphibians.

Relyea RA ，Jones DK 《ENVIRONMENTAL TOXICOLOGY AND CHEMISTRY》

Effects of glyphosate-based herbicides on survival, development, growth and sex ratios of wood frog (Lithobates sylvaticus) tadpoles. II: agriculturally relevant exposures to Roundup WeatherMax® and Vision® under laboratory conditions.

Lanctôt C ，Navarro-Martín L ，Robertson C ，Park B ，Jackman P ，Pauli BD ，Trudeau VL ... -  《-》