The tumor suppressor p33ING1b upregulates p16INK4a expression and induces cellular senescence.

ING1 protein is a tumor suppressor which plays significant roles in multiple cellular activities. p47(ING1a) and p33(ING1b) are major splice isoforms of ING1 and their roles in senescence need further investigations. Here we studied the functions of ING1 isoforms in cellular senescence and gene regulation, with focus on p16(INK4a). We observe that p33(ING1b) protein is the major ING1 isoform expressed in 2BS human diploid fibroblasts. Overexpression of p33(ING1b) induces cellular senescence and upregulates p16(INK4a) expression in 2BS fibroblasts. p33(ING1b) upregulates p16(INK4a) transcription. p33(ING1b) and p300 bind to the p16(INK4a) promoter. p300/CBP-specific inhibitor curcumin can reverse the induction of p16(INK4a) by p33(ING1b). These results help to better understand the function of ING1.

Li N ，Li Q ，Cao X ，Zhao G ，Xue L ，Tong T ... -  《-》

ING1a expression increases during replicative senescence and induces a senescent phenotype.

Soliman MA ，Berardi P ，Pastyryeva S ，Bonnefin P ，Feng X ，Colina A ，Young D ，Riabowol K ... -  《-》

ING1 isoforms differentially affect apoptosis in a cell age-dependent manner.

Vieyra D ，Toyama T ，Hara Y ，Boland D ，Johnston R ，Riabowol K ... -  《CANCER RESEARCH》

ING1 represses transcription by direct DNA binding and through effects on p53.

Kataoka H ，Bonnefin P ，Vieyra D ，Feng X ，Hara Y ，Miura Y ，Joh T ，Nakabayashi H ，Vaziri H ，Harris CC ，Riabowol K ... -  《CANCER RESEARCH》

Subcellular targeting of p33ING1b by phosphorylation-dependent 14-3-3 binding regulates p21WAF1 expression.

Gong W ，Russell M ，Suzuki K ，Riabowol K ... -  《-》