Circulating levels of chromatin fragments are inversely correlated with anti-dsDNA antibody levels in human and murine systemic lupus erythematosus.

Anti-dsDNA antibodies represent a central pathogenic factor in Lupus nephritis. Together with nucleosomes they deposit as immune complexes in the mesangial matrix and along basement membranes within the glomeruli. The origin of the nucleosomes and when they appear e.g. in circulation is not known. Serum samples from autoimmune (NZBxNZW)F1 mice, healthy BALB/c mice, patients with SLE, RA and normal healthy individuals were analyzed for presence and amount of circulating anti-dsDNA antibodies and nucleosomal DNA. Here we use a quantitative PCR to measure circulating DNA in sera. We demonstrate an inverse correlation between anti-dsDNA antibodies and the DNA concentration in the circulation in both murine and human serum samples. High titer of anti-DNA antibodies in human sera correlated with reduced levels of circulating chromatin, and in lupus prone mice with deposition within glomeruli. The inverse correlation between DNA concentration and anti-dsDNA antibodies may reflect antibody-dependent deposition of immune complexes during the development of lupus nephritis in autoimmune lupus prone mice. The measurement of circulating DNA in SLE sera by using qPCR may indicate and detect the development of lupus nephritis at an early stage.

Jørgensen MH ，Rekvig OP ，Jacobsen RS ，Jacobsen S ，Fenton KA ... -  《-》

Pure anti-dsDNA mAbs need chromatin structures to promote glomerular mesangial deposits in BALB/c mice.

Fenton KA ，Tømmerås B ，Marion TN ，Rekvig OP ... -  《-》

Exposure of chromatin and not high affinity for dsDNA determines the nephritogenic impact of anti-dsDNA antibodies in (NZBxNZW)F1 mice.

Mjelle JE ，Kalaaji M ，Rekvig OP 《-》

Critical comparative analyses of anti-alpha-actinin and glomerulus-bound antibodies in human and murine lupus nephritis.

Kalaaji M ，Sturfelt G ，Mjelle JE ，Nossent H ，Rekvig OP ... -  《ARTHRITIS AND RHEUMATISM》

Cross-reactivity of human lupus anti-DNA antibodies with alpha-actinin and nephritogenic potential.

Zhao Z ，Weinstein E ，Tuzova M ，Davidson A ，Mundel P ，Marambio P ，Putterman C ... -  《ARTHRITIS AND RHEUMATISM》