A genetically homogenous population of Fusarium circinatum causes pitch canker of Pinus radiata in the Basque Country, Spain.

Pitch canker, caused by Fusarium circinatum, is a destructive disease of Pinus species and has recently been shown to represent a substantial threat to natural and commercial forests in northern Spain. The genetic diversity of F. circinatum in the Basque Country of Spain was assessed by characterising 96 isolates based on vegetative compatibility groups (VCGs), mating type assays, polymorphic DNA-markers and amplified fragment length polymorphism (AFLP) analyses. For this purpose, F. circinatum isolates were collected from diseased Pinus radiata as well as from insects associated with this host. Overall, a low level of diversity was detected in the population. The isolates represented only two VCGs and they were all of the same mating type. AFLP analyses revealed three genotypes and polymorphic DNA-markers specific for F. circinatum showed nine genotypes. The most common genotypes represented 97% of all isolates for AFLP analysis and 68% of isolates for the polymorphic DNA-marker sets. Over all, this indicates that pitch canker in the Basque Country of Spain is caused by a clonally propagating population of F. circinatum, typical of a recently introduced pathogen.

Iturritxa E ，Ganley RJ ，Wright J ，Heppe E ，Steenkamp ET ，Gordon TR ，Wingfield MJ ... -  《Fungal Biology》

Characterization of Fusarium circinatum from Pinus spp. in northern Spain.

Pérez-Sierra A ，Landeras E ，León M ，Berbegal M ，García-Jiménez J ，Armengol J ... -  《mycological research》

Evidence for multiple introductions and clonality in Spanish populations of Fusarium circinatum.

Berbegal M ，Pérez-Sierra A ，Armengol J ，Grünwald NJ ... -  《-》

The application of high-throughput AFLP's in assessing genetic diversity in Fusarium oxysporum f. sp. cubense.

Groenewald S ，Van Den Berg N ，Marasas WF ，Viljoen A ... -  《mycological research》

Sensitive detection of Fusarium circinatum in pine seed by combining an enrichment procedure with a real-time polymerase chain reaction using dual-labeled probe chemistry.

Ioos R ，Fourrier C ，Iancu G ，Gordon TR ... -  《-》