Interleukin-6 modifies mRNA expression in mouse skeletal muscle.

The aim of this study was to test the hypothesis that interleukin (IL)-6 plays a role in exercise-induced peroxisome proliferator-activated receptor γ co-activator (PGC)-1α and tumor necrosis factor (TNF)-α mRNA responses in skeletal muscle and to examine the potential IL-6-mediated AMP-activated protein kinase (AMPK) regulation in these responses. Whole body IL-6 knockout (KO) and wildtype (WT) male mice (4 months of age) performed 1 h treadmill exercise. White gastrocnemius (WG) and quadriceps (Quad) muscles were removed immediately (0') or 4 h after exercise and from mice not run acutely. Acute exercise reduced only in WT muscle glycogen concentration to 55 and 35% (P < 0.05) of resting level in Quad and WG respectively. While AMPK and Acetyl CoA carboxylase (ACC) phosphorylation increased 1.3-fold (P < 0.05) in WG and twofold in Quad immediately after exercise in WT mice, no change was detected in WG in IL-6 KO mice. The PGC-1α mRNA content was in resting WG 1.8-fold higher (P < 0.05) in WT mice than in IL-6 KO mice. Exercise induced a delayed PGC-1α mRNA increase in Quad in IL-6 KO mice (12-fold at 4 h) relative to WT mice (fivefold at 0'). The TNF-α mRNA content was in resting Quad twofold higher (P < 0.05) in IL-6 KO than in WT, and WG TNF-α mRNA increased twofold (P < 0.05) immediately after exercise only in IL-6 KO. In conclusion, IL-6 affects exercise-induced glycogen use, AMPK signalling and TNF-α mRNA responses in mouse skeletal muscle.

Adser H ，Wojtaszewski JF ，Jakobsen AH ，Kiilerich K ，Hidalgo J ，Pilegaard H ... -  《-》

PGC-1alpha is not mandatory for exercise- and training-induced adaptive gene responses in mouse skeletal muscle.

Leick L ，Wojtaszewski JF ，Johansen ST ，Kiilerich K ，Comes G ，Hellsten Y ，Hidalgo J ，Pilegaard H ... -  《AMERICAN JOURNAL OF PHYSIOLOGY-ENDOCRINOLOGY AND METABOLISM》

Effects of alpha-AMPK knockout on exercise-induced gene activation in mouse skeletal muscle.

Jørgensen SB ，Wojtaszewski JF ，Viollet B ，Andreelli F ，Birk JB ，Hellsten Y ，Schjerling P ，Vaulont S ，Neufer PD ，Richter EA ，Pilegaard H ... -  《-》

PGC-1alpha mediates exercise-induced skeletal muscle VEGF expression in mice.

Leick L ，Hellsten Y ，Fentz J ，Lyngby SS ，Wojtaszewski JF ，Hidalgo J ，Pilegaard H ... -  《-》

PGC-1{alpha} is required for AICAR-induced expression of GLUT4 and mitochondrial proteins in mouse skeletal muscle.

Leick L ，Fentz J ，Biensø RS ，Knudsen JG ，Jeppesen J ，Kiens B ，Wojtaszewski JF ，Pilegaard H ... -  《-》