Analysis of differential gene expression by bead-based fiber-optic array in nonfunctioning pituitary adenomas.

Nonfunctioning pituitary adenomas (NFPAs) are relatively common, accounting for 30% of all pituitary adenomas; however, their pathogenesis remains enigmatic. To explore the possible pathogenesis of NFPAs, we used fiber-optic BeadArray to examine gene expression in 5 NFPAs compared with 3 normal pituitaries. 4 differentially expressed genes were chosen randomly for validation by reverse transcriptase-real time quantitative polymerase chain reaction (RT-qPCR). We then analyzed the differentially expressed gene profile with Kyoto Encyclopedia of Genes and Genomes (KEGG). The array analysis indentified significant increases in the expression of 1,402 genes and 383 expressed sequence tags (ESTs), and decreases in 1,697 genes and 113 ESTs in the NFPAs. Bioinformatic and pathway analysis showed that the genes HIGD1B, FAM5C, PMAIP1 and the pathway cell-cycle regulation may play an important role in tumorigenesis and progression of NFPAs. Our data suggest fiber-optic BeadArray combined with pathway analysis of differential gene expression profile appears to be a valid approach for investigating the pathogenesis of tumors.

Jiang Z ，Gui S ，Zhang Y 《-》

Differential gene expression by fiber-optic beadarray and pathway in adrenocorticotrophin-secreting pituitary adenomas.

Jiang ZQ ，Gui SB ，Zhang YZ 《-》

Analysis of differential gene expression using fiber-optic bead array and pathway analyses in pituitary adenomas.

Pituitary adenomas are commonly occurring, benign neoplasms that account for 15% of intracranial neoplasms but their pathogenesis remains elusive. To explore the possible pathogenesis of pituitary adenomas, we used fiber-optic bead arrays to compare the gene expression profiles in five major pituitary adenoma subtypes to normal pituitary gland from three recently deceased males. We used reverse transcriptase-real time quantitative polymerase chain reaction (RT-qPCR) to randomly validate the expression of four differentially expressed genes in the five pituitary adenoma subtypes and three normal pituitary glands. We then analyzed the profiles of the differentially expressed genes using the Kyoto Encyclopedia of Genes and Genomes pathways. Array analysis showed that 129 genes and two expressed sequence tags were differentially co-expressed in all pituitary adenomas. Pathway analyses showed that the extracellular matrix-receptor interaction pathway and the transforming growth factor-beta signaling pathway may have an important role in tumorigenesis of the five pituitary adenoma subtypes. Thus, we suggest the presence of a common mode of pathogenesis in pituitary adenoma subtypes. Fiber-optic bead array analysis combined with pathway analysis of differentially expressed genes appears to be a valid method of investigating the pathogenesis of different tumors.

Jiang Z ，Gui S ，Zhang Y 《-》

Analysis of differential gene expression in plurihormonal pituitary adenomas using bead-based fiber-optic arrays.

Jiang Z ，Gui S ，Zhang Y 《-》

Differential gene expression profiles of invasive and non-invasive non-functioning pituitary adenomas based on microarray analysis.

Galland F ，Lacroix L ，Saulnier P ，Dessen P ，Meduri G ，Bernier M ，Gaillard S ，Guibourdenche J ，Fournier T ，Evain-Brion D ，Bidart JM ，Chanson P ... -  《-》