Unilateral ureteral obstruction evokes renal tubular apoptosis via the enhanced oxidative stress and endoplasmic reticulum stress in the rat.

Oxidative stress and endoplasmic reticulum (ER) stress may induce renal apoptosis and contribute to the pathogenesis of the kidney with unilateral ureteral obstruction (UUO). We induced UUO the female Wistar rats by ligation of the left ureter at the ureteropelvic junction. The UUO kidney was performed from 4 hr to 7 days course. At the indicated time, we measured the arterial blood pressure and renal blood flow in each rat, renal ROS measurement in vivo by a chemiluminescence analyzer. We performed immunohistochemistry of monocyte/macrophage (ED-1) stain for leukocyte infiltration, 4-hydroxynoneal (4-HNE) stain for ROS products, and apoptosis by terminal deoxynucleotidyl transferase-mediated nick-end labeling (TUNEL) and Western blot to analyze ER stress-associated and apoptosis-related proteins expression in the UUO kidney. We found that UUO decreased renal blood flow and increased renal vascular resistance and renal ROS. UUO decreased renal manganese superoxide dismutase (MnSOD) and catalase protein expression in a time-dependent manner. Increased 4-HNE stain in the renal tubules and ED-1 stain in the renal tubulointerstitial compartment occurred after 4 hr of UUO in the kidney. UUO significantly enhanced ER stress markers like ER stress-response protein 25 and glucose-regulated protein 78 and ER-associated apoptosis proteins, c-JUN NH(2) -terminal kinase, and caspase 12, in the kidney. Subsequently, UUO enhanced renal pro-apoptotic Bax and caspase 3 expression and decreased anti-apoptotic Bcl-2 expression, leading to renal tubular apoptosis. Our data suggest that renal tubular apoptosis induced by oxidative stress and ER stress occurred in the UUO kidney.

Yeh CH ，Chiang HS ，Lai TY ，Chien CT ... -  《-》

Endoplasmic reticulum stress implicated in the development of renal fibrosis.

Chiang CK ，Hsu SP ，Wu CT ，Huang JW ，Cheng HT ，Chang YW ，Hung KY ，Wu KD ，Liu SH ... -  《-》

Ulinastatin inhibits renal tubular epithelial apoptosis and interstitial fibrosis in rats with unilateral ureteral obstruction.

Zhang QF 《-》

Role of Sirolimus in renal tubular apoptosis in response to unilateral ureteral obstruction.

Yang M ，Zhuang YY ，Wang WW ，Zhu HP ，Zhang YJ ，Zheng SL ，Yang YR ，Chen BC ，Xia P ，Zhang Y ... -  《-》

Ginsenoside-Rg1 inhibits endoplasmic reticulum stress-induced apoptosis after unilateral ureteral obstruction in rats.

Li SS ，Ye JM ，Deng ZY ，Yu LX ，Gu XX ，Liu QF ... -  《-》