γ-Tocotrienol inhibits angiogenesis of human umbilical vein endothelial cell induced by cancer cell.

Antiangiogenic therapy mediated by food components is an established strategy for cancer chemoprevention. Growth factors play critical roles in tumor angiogenesis. A conditioned medium containing growth factors from human gastric adenocarcinoma SGC-7901 cell conditioned medium was used as an angiogenic stimulus in this study. The purpose of this study was to evaluate the inhibitory effect and possible mechanism of γ-tocotrienol on tumor angiogenesis. The results showed that γ-tocotrienol (10-40 μmol/L) significantly suppressed proliferation, migration and tube formation of human umbilical vein endothelial cells (HUVECs) induced by SGC-7901 cell conditioned medium in a dose-dependent manner. γ-Tocotrienol (800-1200 μg/egg) also inhibited new blood vessel formation on the growing chick embryo chorioallantoic membrane in a dose-dependent manner. Moreover, the inhibitory effects of γ-tocotrienol on HUVECs were correlated with inducing the apoptosis and arresting cell cycle at the G(0)/G(1) phase at a dose of 40 μmol/L γ-tocotrienol. In addition, γ-tocotrienol inhibited angiogenesis in HUVECs by down-regulation of β-catenin, cyclin D1, CD44, phospho-VEGFR-2 and MMP-9. The antiangiogenic effects of γ-tocotrienol on HUVECs may be attributable to regulation of Wnt signaling by decreasing β-catenin expression. Thus, our results suggest that γ-tocotrienol has a potential chemopreventive agent via antiangiogenesis.

Li Y ，Sun WG ，Liu HK ，Qi GY ，Wang Q ，Sun XR ，Chen BQ ，Liu JR ... -  《-》

Tumor anti-angiogenic effect and mechanism of action of delta-tocotrienol.

Shibata A ，Nakagawa K ，Sookwong P ，Tsuzuki T ，Oikawa S ，Miyazawa T ... -  《-》

Jiedu Xiaozheng Yin, a Chinese herbal formula, inhibits tumor angiogenesis via downregulation of VEGF-A and VEGFR-2 expression in vivo and in vitro.

Cao Z ，Lin W ，Huang Z ，Chen X ，Zhao J ，Zheng L ，Ye H ，Liu Z ，Liao L ，Du J ... -  《-》

gamma-Tocotrienol induces mitochondria-mediated apoptosis in human gastric adenocarcinoma SGC-7901 cells.

Sun W ，Xu W ，Liu H ，Liu J ，Wang Q ，Zhou J ，Dong F ，Chen B ... -  《-》

Y-tocotrienol inhibits angiogenesis-dependent growth of human hepatocellular carcinoma through abrogation of AKT/mTOR pathway in an orthotopic mouse model.

Siveen KS ，Ahn KS ，Ong TH ，Shanmugam MK ，Li F ，Yap WN ，Kumar AP ，Fong CW ，Tergaonkar V ，Hui KM ，Sethi G ... -  《Oncotarget》