Mycelial growth and solid-state fermentation of lignocellulosic waste by white-rot fungus Phanerochaete chrysosporium under lead stress.

Lignocellulosic biomass is an abundant renewable resource difficult to degrade. Its bioconversion plays important roles in carbon cycles in nature, which may be influenced by heavy metals in environment. Mycelial growth and the degradation of lignocellulosic waste by lignin-degrading fungus Phanerochaete chrysosporium under lead stress were studied. It was shown that P. chrysosporium could grow in liquid media with 400 mg L⁻¹ Pb(II), and mycelial dry weight was reduced by 54% compared to the control. Yellow mycelia in irregular short-strip shape formed in Pb-containing media, whereas the control showed ivory-white regular mycelial pellets. Two possible responses to Pb stress were: dense hyphae, and secretion from mycelia to resist Pb. During solid-state fermentation of straw, fungal colonization capability under Pb stress was positively correlated with the removal efficiency of soluble-exchangeable Pb when its content was higher than 8.2 mg kg⁻¹ dry mass. Carboxymethyl cellulase activity and cellulose degradation were inhibited at different Pb concentrations, whereas low Pb concentrations increased xylanase and ligninolytic enzyme activities and the hemicellulose and lignin degradation. Cluster analyses indicated that Pb had similar effects on the different microbial indexes related to lignin and hemicellulose degradation. The present findings will advance the understandings of lignocellulose degradation by fungi under Pb pollution, which could provide useful references for developing metal-polluted waste biotreatment technology.

Huang DL ，Zeng GM ，Feng CL ，Hu S ，Zhao MH ，Lai C ，Zhang Y ，Jiang XY ，Liu HL ... -  《-》

Oxalate production at different initial Pb²+ concentrations and the influence of oxalate during solid-state fermentation of straw with Phanerochaete chrysosporium.

The production of oxalate at different initial Pb(2+) concentrations during solid-state fermentation of straw with Phanerochaete chrysosporium was investigated. It was found that the maximal peak value of oxalate concentration (22.84 mM) was detected at the initial Pb(2+) concentration of 200 mg kg(-1) dry straw, while the minimum (15.89 mM) at the concentration of 600 mg Pb(2+)kg(-1) dry straw, and at moderate concentration of Pb(2+) the capability of oxalic acid secretion was enhanced. In addition, it was also found that more oxalic acid accumulation went together with better Pb(2+) passivation effect and higher manganese peroxidase (MnP) activity. The present findings will improve the understandings of the interactions of heavy metals with white-rot fungi and the role of oxalate in lignin degradation system, which could provide useful references for more efficient treatment of Pb-contaminated lignocellulosic waste.

Li NJ ，Zeng GM ，Huang DL ，Hu S ，Feng CL ，Zhao MH ，Lai C ，Huang C ，Wei Z ，Xie GX ... -  《-》

Degradation of lead-contaminated lignocellulosic waste by Phanerochaete chrysosporium and the reduction of lead toxicity.

Huang DL ，Zeng GM ，Feng CL ，Hu S ，Jiang XY ，Tang L ，Su FF ，Zhang Y ，Zeng W ，Liu HL ... -  《-》

Biodegradation of lignin and nicotine with white rot fungi for the delignification and detoxification of tobacco stalk.

Su Y ，Xian H ，Shi S ，Zhang C ，Manik SM ，Mao J ，Zhang G ，Liao W ，Wang Q ，Liu H ... -  《BMC BIOTECHNOLOGY》

[Comparison of lignocellulolytic enzyme profiles secreted by Panus conchatus and Phanerochaete chrysosporium during solid state cultures].

Wang C ，Yu H ，Fu S 《-》