Establishment of a mouse IgA nephropathy model with the MBP-20-peptide fusion protein.

Here, we aimed to determine whether immunoglobulin-A nephropathy (IgAN) could be induced in Balb/c mice by immunizing them with a fusion protein (MBP-20 peptide) comprising the maltose-binding protein (MBP) and a 20-amino-acid peptide derived from Staphylococcus aureus. A recombinant plasmid encoding the fusion protein was constructed and expressed in bacterial cells. The synthetic 20-peptide was used to prepare the monoclonal antibody. Balb/c mice were immunized with the MBP-20-peptide fusion protein over a 21-week course before renal histology was examined at the light and electron microscopic levels. Direct immunofluorescence staining with the anti-20-peptide monoclonal antibody was also performed using renal biopsy tissue from human IgAN patients as a comparison. IgA and IgG specific for the 20-peptide in human and mice serum were detected. The IgAN experimental mice developed a clinical and pathological profile that closely resembled that of human IgAN patients, including the induction of hematuria and numerous histopathological features. Levels of IgA and IgG specific for the 20-peptide were significantly increased in serum from the IgAN experimental mice and IgAN patients compared with control mice and non-IgAN patients. In IgAN model mice, the anti-20-peptide antibody labeled glomeruli, while the antibody strongly labeled glomeruli and weakly labeled tubular epithelial cells in renal tissue from human IgAN patients. In conclusion, immunization with an MBP-20-peptide fusion protein is able to induce clinical and pathological features closely resembling IgAN in Balb/c mice, indicating a potentially useful role for the model in the study of IgAN and related diseases.

Zhang L ，Ye F ，He Y ，Kong D ，Han C ，Zhao Z ，Zhu J ，Meng H ，Liu X ，Jin X ... -  《-》

Staphylococcus aureus antigens induce IgA-type glomerulonephritis in Balb/c mice.

Sharmin S ，Shimizu Y ，Hagiwara M ，Hirayama K ，Koyama A ... -  《JOURNAL OF NEPHROLOGY》

Anti-macrophage migration inhibitory factor reduces transforming growth factor-beta 1 expression in experimental IgA nephropathy.

Leung JC ，Chan LY ，Tsang AW ，Liu EW ，Lam MF ，Tang SC ，Lai KN ... -  《NEPHROLOGY DIALYSIS TRANSPLANTATION》

The role of mesangial complement in the hematuria of experimental IgA nephropathy.

Emancipator SN ，Ovary Z ，Lamm ME 《LABORATORY INVESTIGATION》

Experimental IgA-IgG nephropathy induced by a viral respiratory pathogen. Dependence on antigen form and immune status.

Jessen RH ，Emancipator SN ，Jacobs GH ，Nedrud JG ... -  《LABORATORY INVESTIGATION》