Asymmetric response patterns to subspecies-specific song differences in allopatry and parapatry in the gray-breasted wood-wren.

Song divergence between closely related taxa may play a critical role in the evolutionary processes of speciation and hybridization. We explored song variation between two Ecuadorian subspecies of the gray-breasted wood-wren (Henicorhina leucophrys) and tested the impact of song divergence on response behaviors. Songs were significantly different between the two subspecies, even between two parapatric populations 10 km apart. Playback experiments revealed an asymmetric response pattern to these divergent subspecies specific songs; one subspecies responded more to songs of its own subspecies than to the other subspecies' songs, whereas the second responded equally strongly to songs of both subspecies. While song parameters revealed a mixed pattern of divergence between allopatric and parapatric populations, the majority of spectral characteristics showed increased divergence in parapatry, suggestive of character displacement. This increased song divergence in parapatry appeared to affect behavioral responses to playback as discriminating responses were most prominent in parapatry and against parapatric songs. The clear behavioral impact of subspecies-specific song differences supports a potential role for song as an acoustic barrier to gene flow. The asymmetric nature of the responses suggests that song divergence could affect the direction of gene flow and the position of the subspecies-specific transition.

Dingle C ，Poelstra JW ，Halfwerk W ，Brinkhuizen DM ，Slabbekoorn H ... -  《-》

Habitat-dependent song divergence at subspecies level in the grey-breasted wood-wren.

Dingle C ，Halfwerk W ，Slabbekoorn H 《-》

Sharp acoustic boundaries across an altitudinal avian hybrid zone despite asymmetric introgression.

Birdsong is a sexually selected trait that could play an important evolutionary role when related taxa come into secondary contact. Many songbird species, however, learn their songs through copying one or more tutors, which complicates the evolutionary outcome of such contact. Two subspecies of a presumed vocal learner, the grey-breasted wood-wren (Henicorhina leucophrys), replace each other altitudinally across the western slope of the Ecuadorian Andes. These subspecies are morphologically very similar, but show striking differences in their song. We examined variation in acoustic traits and genetic composition across the altitudinal range covered by both subspecies and between two allopatric populations. The acoustic boundary between the subspecies was found to be highly abrupt across a narrow elevational range with virtually no evidence of song convergence. Mixed singing and use of hetero-subspecific song occurred in the contact zone and was biased towards the use of leucophrys song types. Hetero-subspecific song copying by hilaris and not by leucophrys reflected a previously found asymmetric pattern of response to song playback. Using amplified fragment length polymorphisms (AFLP) markers, we detected hybridization in the contact zone and asymmetric introgression in parapatric populations, with more leucophrys alleles present in hilaris populations than vice versa. This pattern may be a trail of introgression due to upslope displacement of leucophrys by hilaris. Our data suggest that song learning may impact speciation and hybridization in contrasting ways at different spatial scales: although learning may speed up population divergence in songs, thereby enhancing assortative mating and reducing gene flow, it may at a local level also lead to the copying of heterospecific songs, therefore allowing some level of hybridization and introgression.

Halfwerk W ，Dingle C ，Brinkhuizen DM ，Poelstra JW ，Komdeur J ，Slabbekoorn H ... -  《-》

Asymmetric Response of Costa Rican White-Breasted Wood-Wrens (Henicorhina leucosticta) to Vocalizations from Allopatric Populations.

Pegan TM ，Rumelt RB ，Dzielski SA ，Ferraro MM ，Flesher LE ，Young N ，Class Freeman A ，Freeman BG ... -  《PLoS One》

A preference for own-subspecies' song guides vocal learning in a song bird.

:In many song birds, males develop their songs as adults by imitating the songs of one or more tutors, memorized previously during a sensitive phase early in life. Previous work using two assays, the production of imitations by adult males and playback-induced calling by young birds during the sensitive phase for memorization, has shown that song birds can discriminate between their own and other species' songs. Herein I use both assays to show that male mountain white-crowned sparrows, Zonotrichia leucophrys oriantha, must learn to sing but have a genetic predisposition to memorize and learn the songs of their own subspecies. Playback tests to young naive birds before they even begin to sing reveal that birds give begging calls more in response to oriantha song than to songs of another species. After 10 days of tutoring with songs of either their own or another subspecies, birds continue to give stronger call responses to songs of their own subspecies, irrespective of whether they were tutored with them, and are more discriminating in distinguishing between different dialects of their own subspecies. The memory processes that facilitate recognition and discrimination of own-subspecies' song may also mediate the preferential imitation of song of a bird's own subspecies. Such perceptual biases could constrain the direction and rate of cultural evolution of learned songs.

Nelson DA 《PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA》