Epidemiology and molecular characteristics of community-associated methicillin-resistant and methicillin-susceptible Staphylococcus aureus from skin/soft tissue infections in a children's hospital in Beijing, China.

To evaluate the epidemiology and molecular features of community-associated methicillin-resistant Staphylococcus aureus (CA-MRSA) and methicillin-sensitive S. aureus (MSSA) from children with skin and soft tissue infections (SSTIs) in Beijing, China, prospective community-acquired S. aureus SSTIs surveillance was conducted at the Beijing Children's Hospital, Beijing, China, for a 12-month period from August 1, 2008, to July 30, 2009. Susceptibility to 12 antimicrobials was determined by the agar dilution method. Genotypic characteristics of CA-MRSA isolates were tested by SCCmec typing, spa typing, and multilocus sequence typing. Panton-Valentine leukocidin gene was detected. Of 1104 cases, 31.8% (351) were community-acquired S. aureus. CA-MRSA accounted for 4% (14) of S. aureus. Among 14 CA-MRSA and 120 MSSA isolates tested, 100% and 91.7% were multidrug resistant, respectively. ST59-MRSA-IVa-t437 (42.9%) was the most common form of CA-MRSA. Spa typing analysis of 120 MSSA isolates was performed, followed by pulsed-field gel electrophoresis and multilocus sequence typing of a selected number of isolates. The most common spa types among MSSA were t084 (8.3%), t091 (5.8%), t034 (5%), t127 (4.2%), t002 (4.2%), and t796 (4.2%). No predominant spa type was seen. Of the MSSA isolates that could be classified into spa-CCs, 15.0% had a genetic background observed in CA-MRSA clones (spa-CC437, spa-CC342, and spa-CC377). Panton-Valentine Leukocidin (PVL)-positive community-acquired S. aureus strains were more commonly associated with skin abscesses than other SSTIs (29.4% versus 5.9%, P < 0.01).In conclusion, CA-MRSA infections are not common among Chinese children with SSTIs. Our findings show that MSSA strains in China have diverse genetic backgrounds.

Wu D ，Wang Q ，Yang Y ，Geng W ，Wang Q ，Yu S ，Yao K ，Yuan L ，Shen X ... -  《-》

Community-associated methicillin-resistant and methicillin-sensitive Staphylococcus aureus: skin and soft tissue infections in Hong Kong.

Ho PL ，Chuang SK ，Choi YF ，Lee RA ，Lit AC ，Ng TK ，Que TL ，Shek KC ，Tong HK ，Tse CW ，Tung WK ，Yung RW ，Hong Kong CA-MRSA surveillance network ... -  《DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE》

Molecular characterization and susceptibility of methicillin-resistant and methicillin-susceptible Staphylococcus aureus isolates from hospitals and the community in Vladivostok, Russia.

Baranovich T ，Zaraket H ，Shabana II ，Nevzorova V ，Turcutyuicov V ，Suzuki H ... -  《-》

Characterization of community-associated Staphylococcus aureus from skin and soft-tissue infections: a multicenter study in China.

Liu Y ，Xu Z ，Yang Z ，Sun J ，Ma L ... -  《Emerging Microbes & Infections》

Skin and soft tissue infections caused by community-associated methicillin-resistant staphylococcus aureus among children in China.

Geng W ，Yang Y ，Wang C ，Deng L ，Zheng Y ，Shen X ... -  《-》