Expression changes of K+-Cl- co-transporter 2 and Na+-K+-Cl- co-transporter1 in mouse trigeminal subnucleus caudalis following pulpal inflammation.

Cation chloride co-transporters, including K(+)-Cl(-) co-transporter 2 (KCC2) and Na(+)-K(+)-Cl(-) co-transporter 1 (NKCC1), are of particular importance to GABAergic transmission and thus involved in the development of hyperalgesia at the spinal level. However, it is largely unknown whether these co-transporters in the trigeminal system contribute to dental pain. In this study, we investigated the expression of KCC2 and NKCC1 mRNAs in mouse trigeminal subnucleus caudalis (Vc) after lipopolysaccharide (LPS) application to the tooth pulp by real-time reverse transcriptase-polymerase chain reaction (RT-PCR) method. KCC2 mRNA was found to be down-regulated at 1d after pulpal inflammation, while NKCC1 was up-regulated. Blockade of endogenous brain-derived neurotrophic factor-tyrosine receptor kinase B pathway with K252a produced pronounced antinociception as evidenced by decreased tongue protrusion behavior in LPS-treated mice. These data suggest that KCC2 and NKCC1 in Vc may play a critical role in the nociception and transmission of dental pain during pulpal inflammation.

Li YQ ，Li H ，Wei J ，Qu L ，Wu LA ... -  《-》

Down-regulation of K+ -Cl- co-transporter 2 in mouse medullary dorsal horn contributes to the formalin-induced inflammatory orofacial pain.

Wu LA ，Huang J ，Wang W ，Wang W ，Wang XJ ，Wu SX ... -  《-》

Reduced potassium-chloride co-transporter expression in spinal cord dorsal horn neurons contributes to inflammatory pain hypersensitivity in rats.

Zhang W ，Liu LY ，Xu TL 《NEUROSCIENCE》

Differential functional expression of cation-Cl- cotransporter mRNAs (KCC1, KCC2, and NKCC1) in rat trigeminal nervous system.

Toyoda H ，Yamada J ，Ueno S ，Okabe A ，Kato H ，Sato K ，Hashimoto K ，Fukuda A ... -  《-》

Long-term expressional changes of Na+ -K+ -Cl- co-transporter 1 (NKCC1) and K+ -Cl- co-transporter 2 (KCC2) in CA1 region of hippocampus following lithium-pilocarpine induced status epilepticus (PISE).

Li X ，Zhou J ，Chen Z ，Chen S ，Zhu F ，Zhou L ... -  《BRAIN RESEARCH》