Peroxisome proliferator-activated receptor-gamma (PPAR-gamma) agonist inhibits transforming growth factor-beta1 and matrix production in human dermal fibroblasts.

Peroxisome proliferator-activated receptor-gamma (PPAR-gamma) agonists are increasingly used in patients with diabetes, and some studies have suggested a beneficial effect on organ fibrosis, but their effects on dermal cell growth and extracellular matrix (ECM) turnover are unknown. To investigate the effect of the PPAR-gamma agonist troglitazone on cell growth and matrix production in human dermal fibroblasts (HDF), HDF were cultured and grown in a different concentration of troglitazone. PPAR-gamma expression and matrix production were measured in HDF in the presence of troglitazone. The mRNA expressions of TGF-beta1, collagen I (Col I) and fibronectin (FN) were determined by quantitative real-time reverse transcription polymerase chain reaction (RT-PCR). The protein of transforming growth factor-beta1 (TGF-beta1) was determined by enzyme-linked immunosorbent assay (ELISA) and proteins of Col I and FN were determined by Western blotting. The mRNA expression of TGF-beta1, Col I and FN were significantly decreased in HDF in 15-30 micromol l(-1) troglitazone compared to the control group with Dulbecco's modified Eagle's medium (P<0.01). An obvious decrease of TGF-beta1 protein was found in troglitazone-treated groups as compared to the control group (P<0.05). Exposure of HDF to troglitazone reduced col I secretion (P<0.05), and fibronectin secretion (P<0.05). This study suggests that PPAR-gamma agonist will provide a novel approach with therapeutic potential in dermal fibrosis, such as hypertrophic scar, keloid and so on.

Zhang GY ，Cheng T ，Zheng MH ，Yi CG ，Pan H ，Li ZJ ，Chen XL ，Yu Q ，Jiang LF ，Zhou FY ，Li XY ，Yang JQ ，Chu TG ，Gao WY ... -  《-》

Troglitazone inhibits synthesis of transforming growth factor-beta1 and reduces matrix production in human peritoneal mesothelial cells.

Peng Y ，Liu H ，Liu F ，Liu Y ，Li J ，Chen X ... -  《NEPHROLOGY》

Troglitazone suppresses transforming growth factor-beta1-induced collagen type I expression in keloid fibroblasts.

Zhang GY ，Yi CG ，Li X ，Ma B ，Li ZJ ，Chen XL ，Guo SZ ，Gao WY ... -  《-》

Pioglitazone inhibits cell growth and reduces matrix production in human kidney fibroblasts.

Zafiriou S ，Stanners SR ，Saad S ，Polhill TS ，Poronnik P ，Pollock CA ... -  《JOURNAL OF THE AMERICAN SOCIETY OF NEPHROLOGY》

A selective cyclooxygenase-2 inhibitor decreases transforming growth factor-beta1 synthesis and matrix production in human peritoneal mesothelial cells.

Liu H ，Peng Y ，Liu F ，Li J ，Chen X ，Liu Y ，Zhang H ... -  《CELL BIOLOGY INTERNATIONAL》