Delivery rate using cryopreserved oocytes is comparable to conventional in vitro fertilization using fresh oocytes: potential fertility preservation for female cancer patients.

To explore the use of oocyte cryopreservation as a fertility-conserving option. Cancer treatments administered during the reproductive and adolescent years can result in sterility. Previous fertility preservation efforts focused on embryo rather than oocyte storage because the latter was deemed inefficient. Recently, several large reports of healthy births resulting from the transfer of embryos derived from frozen/thawed oocytes have been published. We sought to establish an oocyte cryopreservation program at our center. Twenty-three oocyte cryopreservation cycles were performed. Collected oocytes were cryopreserved by either the slow or the vitrification method. Approximately 1-4 months later, a programmed cycle of thawing/warming, fertilization with intracytoplasmic sperm injection, and ET was performed; cycle and pregnancy outcomes were assessed. University-based fertility center. Twenty-two infertile women. Oocyte cryopreservation. Oocyte survival, embryo development, pregnancy outcomes. Oocyte survival, 2-pronuclei fertilization, and blastocyst formation rates were 92%, 79%, and 43%, respectively. Fourteen women became pregnant; one miscarried; 10 have delivered 13 viable infants, and three pregnancies are ongoing for an ongoing/delivered pregnancy rate of 57%. This result was not statistically different from cycles performed consecutively in age-matched controls using fresh, nonfrozen autologous or donor oocytes during a similar time period. Oocyte cryopreservation appears to be a viable option for fertility preservation in some centers.

Grifo JA ，Noyes N 《-》

[Effects of cryopreservation time and thawing method of human oocyte vitrification on the outcome of assisted reproduction].

Song WY ，Sun YP ，Jin HX ，Xin ZM ，Su YC ，Guo YH ，Chen ZJ ... -  《-》

Efficiency of oocyte cryopreservation: a meta-analysis.

Oktay K ，Cil AP ，Bang H 《-》

Vitrification of oocytes produces high pregnancy rates when carried out in fertile women.

To determine the efficiency of our vitrification technique when applied in young fertile women. Clinical research and application. In vitro fertilization center. Twenty-one women were recruited from the navy community with 19 patients finishing the study. Vitrified oocytes with use of the electron microscopic grid method were warmed 6 months after vitrification. Surviving metaphase II oocytes were microinjected for fertilization, and clinical results were evaluated. Survival, fertilization, and cleavage rate. Pregnancy and implantation rate. Three hundred ninety-five oocytes were warmed, of which 320 oocytes (81.0%) survived. Two hundred eighty-five metaphase II oocytes were microinjected for fertilization; 206 of them (72.3%) fertilized, and 53 embryos were transferred to 19 patients (in 20 warming cycles). Twenty-four of 53 transferred embryos (45.3%) implanted as confirmed by ultrasound examination. Of the 20 transfers, 16 resulted in clinical pregnancy (80%), 3 miscarried (15%), and 13 (65%) went on to produce 20 live births, respectively. This is much higher in comparison with our previous data using supernumerary oocytes where the rates of implantation and pregnancy were 6% and 21%. Live-birth rates per warmed oocyte and per injected oocyte were 5.1% and 7.2%, respectively. High pregnancy and implantation rates were observed after 6 months of cryopreservation by vitrification when oocytes from fertile woman were used. Proper screening of candidates for oocyte cryopreservation is of crucial importance to assure a favorable pregnancy outcome.

Kim TJ ，Laufer LR ，Hong SW 《-》

[Clinical effects of oocyte cryopreservation in assisted reproduction technology].

Wang W ，Yan ZJ ，Cai LB ，Chai DC ，Liu CZ ，Mao YD ，Liu JY ... -  《-》