Presence of thrombin-activatable fibrinolysis inhibitor in Helicobacter pylori-associated gastroduodenal disease.

Thrombin-activatable fibrinolysis inhibitor (TAFI) plays a role in the regulation of coagulation and inflammation. In addition to inhibiting the fibrinolytic system, TAFI may also regulate the bradykinin and complement systems. We hypothesized that TAFI also plays a role in defense mechanisms of the gastric mucosa during Helicobacter pylori infection. This study comprised 65 patients with gastroduodenal disorders: 41 patients with H. pylori infection, 13 without, and 11 patients with cured H. pylori infection. The gastric intramucosal concentrations of TAFI were measured by enzyme immunoassay. The gastric levels of TAFI and plasminogen activator inhibitor-1 were significantly increased in patients with H. pylori compared to those without infection or cured H. pylori. The presence of TAFI was detected in gastric mucosal epithelial cells. The concentration of TAFI was correlated with the degree of gastric mucosal atrophy, inflammation, and disease activity. These results show that TAFI is present in the gastric mucosa and that it may play a role in the pathogenesis of H. pylori infection-associated gastroduodenal disorders.

Ikeda A ，Gabazza EC ，Morser J ，Imoto I ，Kuroda M ，D'Alessandro-Gabazza CN ，Hara K ，Ruiz DB ，Bernabe PG ，Katsurahara M ，Toda M ，Kobayashi Y ，Yano Y ，Sumida Y ，Suzuki K ，Taguchi O ，Takei Y ... -  《-》

Changes in coagulation-fibrinolysis balance in blood mononuclear cells and in gastric mucosa from patients with Helicobacter pylori infection.

Helicobacter pylori and some of its virulence factors stimulate human blood mononuclear cells (MNC) in vitro to produce tissue factor (TF) and plasminogen activator inhibitor-2 (PAI-2). In this study we investigated the procoagulant-fibrinolytic potential of blood MNC in patients with H. pylori infection. In the same patients we also evaluated the coagulation-fibrinolysis profile in gastric tissue and in plasma. The production of TF and PAI-2 was evaluated in 61 patients with dyspepsia, 31 positive and 30 negative for H. pylori infection. TF expressed by MNC and PAI-2 accumulation in cell culture medium after incubation for 20 h at 37 degrees C were significantly higher in H. pylori(+) than in H. pylori(-) patients and were significantly correlated. TF and PAI-2 content in extracts of gastric mucosa was similar in the two groups whereas lower levels of tissue plasminogen activator (t-PA) and thrombomodulin (TM) antigens were found in the antrum of H. pylori(+) patients. No difference between the groups was observed in plasma thrombus precursor protein, prothrombin fragment 1+2, D-dimer, t-PA, PAI-1, TM and thrombin activatable fibrinolysis inhibitor. H. pylori infection is associated with functional abnormalities of blood MNC resulting in the coordinate expression of TF and antifibrinolytic activity. Changes in cell coagulation-fibrinolysis balance may represent a link between H. pylori infection and ischemic heart disease.

Colucci M ，Rossiello MR ，Pentimone A ，Berloco P ，Russo F ，Di Leo A ，Semeraro N ... -  《THROMBOSIS RESEARCH》

Role of Helicobacter pylori infection in gastroduodenal injury and gastric prostaglandin synthesis during long term/low dose aspirin therapy: a prospective placebo-controlled, double-blind randomized trial.

Feldman M ，Cryer B ，Mallat D ，Go MF ... -  《AMERICAN JOURNAL OF GASTROENTEROLOGY》

Vacuolating cytotoxin A is associated with increased thrombin generation in gastric mucosa.

Suzuki Y ，Gabazza EC ，Imoto I ，James RF ，Hirayama T ，Wada A ，Horiki N ，Nakamura M ，Inoue H ，Kuroda M ，Ogura A ，Taguchi Y ，Yano Y ，Taguchi O ，Suzuki K ，Adachi Y ... -  《HELICOBACTER》

New acquisitions in Helicobacter pylori infection. Introduction.

Gasbarrini G ，Gasbarrini A 《italian journal of gastroenterology and hepatology》