Evaluation of the immature human oocytes from unstimulated cycles in polycystic ovary syndrome patients using a novel scoring system.

To establish a novel scoring system for human immature oocytes and estimate its prognostic effects on the in vitro maturation (IVM) and developmental potential of human immature oocytes. Prospective study. Hospital-based IVF center. Patients with polycystic ovary syndrome (PCOS) undergoing unstimulated cycles of oocyte retrieval. Immature (germinal vesicle and metaphase I) oocytes were collected from PCOS patients and were evaluated based on morphologic characters. After IVM, the maturation rate, fertilization rate, and cleavage rate were evaluated. The quality of the embryos and the apoptosis of the granulosa cells (GCs) were analyzed as well. Morphologic characters of immature oocytes and maturation rate, fertilization rate, cleavage rate, high-quality embryo formation rate, and the apoptosis of the GCs after IVM. The maturation rate, fertilization rate, cleavage rate, and high-quality embryo formation rate of both MI and GV oocytes reduced with the decrease of the score. There were no differences in maturation, fertilization, and cleavage rates between the MI and GV phase. The apoptosis rate of GCs of grade 1' and 0' oocytes was obvious higher than those of grade 3-4'. Evaluating cumulus layer around the oocyte was a simple but useful method, which played an important role in terms of yielding good prognostic parameters in an embryology laboratory. The scoring system of immature oocytes can correctly reflect the development potential of human oocytes.

Liu S ，Jiang JJ ，Feng HL ，Ma SY ，Li M ，Li Y ... -  《-》

Exposure to human chorionic gonadotropin during in vitro maturation does not improve the maturation rate and developmental potential of immature oocytes from patients with polycystic ovary syndrome.

Ge HS ，Huang XF ，Zhang W ，Zhao JZ ，Lin JJ ，Zhou W ... -  《-》

Supplemented tissue culture medium 199 is a better medium for in vitro maturation of oocytes from women with polycystic ovary syndrome women than human tubal fluid.

de Araujo CH ，Nogueira D ，de Araujo MC ，Martins Wde P ，Ferriani RA ，dos Reis RM ... -  《-》

Follicular fluid levels of anti-Mullerian hormone as a predictor of oocyte maturation, fertilization rate, and embryonic development in patients with polycystic ovary syndrome.

To test whether the follicular fluid (FF) concentration of anti-Mullerian hormone (AMH) is associated with oocyte maturation, fertilization rate, and embryonic development in patients with polycystic ovary syndrome (PCOS) undergoing IVF. Prospective. Academic assisted reproductive technology program. Twenty-two samples of FF from 11 patients with PCOS who underwent IVF/ET were analyzed for AMH levels (group A). Twelve women with normal ovulatory cycles served as a control group (group B). The oocytes and preembryos were closely followed until ET. FF was obtained at oocyte retrieval for IVF/intracytoplasmic sperm injection. AMH levels were studied using immunoenzymometric assay. None. FF, AMH levels, oocyte maturation, fertility rate, and embryonic cleavage rate. The mean FF AMH level was 57.3 +/- 79.5 pmol/mL in group A, compared with 70 +/- 120.14 pmol/mL in group B. In group A, the mean AMH level of good-quality embryos was 37.4 +/- 23.4 pmol/mL, compared with 61.9 +/- 102 pmol/mL in a the poor-quality subgroup. No significant correlation was observed between FF AMH levels and oocyte maturation, fertilization, or cleavage rate. This study suggests that there is an association between FF AMH levels and the quality of embryos in patients with PCOS. However, owing to large variance and the number of participants, no statistical significance was reached. The degree of maturation of retrieved oocytes, as well as the success of fertilization, was not found to correlate with FF AMH.

Mashiach R ，Amit A ，Hasson J ，Amzalzg S ，Almog B ，Ben-Yosef D ，Lessing JB ，Limor R ，Azem F ... -  《-》

[In vitro maturation and fertilization of oocytes from unstimulated cycles in women with infertility due to polycystic ovary syndrome].

Li Y ，Jiang JJ ，Ma SY ，Li M ，Hu JM ，Zhao LX ，Chen ZJ ... -  《-》