Ginkgo biloba extract prevents glucose-induced accumulation of ECM in rat mesangial cells.

Pathological remodeling characterized by extracellular matrix (ECM) accumulation contributes to diabetic nephropathy (DN). This study evaluated the effects of Ginkgo biloba extract (GbE) on the metabolism of the ECM in rat mesangial cells cultured in hyperglycemic conditions. The cultured mesangial cells in high glucose conditions were allotted into six groups: normal control group, high glucose group, low concentration of GbE group, moderate concentration of GbE group, high concentration of GbE group, and captopril group. In the presence of high glucose, the levels of matrix metalloproteinase-2 (MMP-2), matrix metalloproteinase-9 (MMP-9) and extracellular matrix metalloproteinase inducer (EMMPRIN) were decreased significantly, and the levels of tissue inhibitor of metalloproteinase-2 (TIMP-2), tissue inhibitor of metalloproteinase-1 (TIMP-1) and plasminogen activator inhibitor-1 (PAI-1) were increased significantly. These changes were reversed by GbE. GbE lowered the levels of transforming growth factor-beta(1) (TGF-beta(1)), insulin-like growth factor-1 (IGF-1) and connective tissue growth factor (CTGF) of the high glucose group. Furthermore, GbE also decreased the expressions of collagen IV and laminin of the high glucose group. In summary, the results suggest that GbE postpones the extracellular matrix accumulation by inhibiting the synthesis of ECM and promoting the degradation of ECM, and therefore, is a potential drug for the prevention and treatment of DN.

Ji L ，Yin XX ，Wu ZM ，Wang JY ，Lu Q ，Gao YY ... -  《-》

Protective effects of serum containing Ginkgo biloba extract on glomerulosclerosis in rat mesangial cells.

Tang D ，Zhang Z ，Gao Y ，Wei Y ，Han L ... -  《-》

Ginkgo biloba extract suppresses hypertrophy and extracellular matrix accumulation in rat mesangial cells.

Wang JY ，Yin XX ，Wu YM ，Tang DQ ，Gao YY ，Wan MR ，Hou XY ，Zhang B ... -  《ACTA PHARMACOLOGICA SINICA》

An imbalance between matrix metalloproteinase-2 and tissue inhibitor of matrix metalloproteinase-2 contributes to the development of early diabetic nephropathy.

Han SY ，Jee YH ，Han KH ，Kang YS ，Kim HK ，Han JY ，Kim YS ，Cha DR ... -  《NEPHROLOGY DIALYSIS TRANSPLANTATION》

Angiotensin-II and rosuvastatin influence matrix remodeling in human mesangial cells via metalloproteinase modulation.

Solini A ，Rossi C ，Santini E ，Madec S ，Salvati A ，Ferrannini E ... -  《-》